DNA aptamer S11e recognizes fibrosarcoma and acts as a tumor suppressor.
DNA aptamer S11e recognizes fibrosarcoma and acts as a tumor suppressor.
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DOI：
10.1016/j.bioactmat.2021.10.011
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发表时间：
2022-06
影响因子：
18.9
通讯作者：
Tan W
中科院分区：
文献类型：
--
作者：
Liu Y;Peng C;Zhang H;Li J;Ou H;Sun Y;Wen C;Qi D;Hu X;Wu E;Tan W
Fibrosarcoma is a serious malignant mesenchymal tumor with strong invasiveness, high recurrence, and poor prognosis. Currently, surgical resection is the main treatment for fibrosarcoma. However, due to the lack of specific biomarkers, the inability to accurately diagnose fibrosarcoma can lead to sub-optimal surgical outcomes and decreased survival. Here, we seek to address this translational barrier and we show that DNA aptamer S11e was able to recognize fibrosarcoma cells (HT1080) but not human embryonic lung fibroblast cells with Kd values in the nanomolar range. In addition, we found that S11e discerned tumors in HT1080 xenograft mouse models and tumor tissues from fibrosarcoma patients. Furthermore, we demonstrated that S11e internalized into HT1080 cells independent of the lysosome pathway and located in mitochondria. Moreover, we revealed that S11e promoted the apoptosis of HT1080 cells and inhibited HT1080 cell migration. Finally, we investigated the biologically functional cellular target of S11e using a mass spectrometry approach, and identified that Diablo/SMAC protein is a cellular binding protein of S11e, by interacting to which S11e inhibited HT1080 cell migration and invasion. Taken together, these results provide the evidence that S11e may be useful for early diagnosis, targeted therapy, and prognostication of fibrosarcoma. S11e specifically targets fibroscarcoma cells and could be a novel tool for the early diagnosis and therapy of fibrosarcoma. S11e can be internalized into HT1080 fibrosarcoma cells and located in mitochondria, which induces the apoptosis of the cells. S11e inhibits fibrosarcoma cell proliferation and migration via binding to Diablo/SMAC protein in mitochondria.
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影响因子：
12.4
作者：
Wu X;Chen J;Wu M;Zhao JX
通讯作者：
Zhao JX
影响因子：
17.1
作者：
Qu H;Csordas AT;Wang J;Oh SS;Eisenstein MS;Soh HT
通讯作者：
Soh HT
影响因子：
4.3
作者：
Shoshan-Barmatz, Varda;Keinan, Nurit;Aram, Lior
通讯作者：
Aram, Lior
影响因子：
14.9
作者：
Troisi R;Napolitano V;Spiridonova V;Russo Krauss I;Sica F
通讯作者：
Sica F
DOI：
10.1093/nass/nrn031
发表时间：
2008-01-01
期刊：
Nucleic acids symposium series (2004)
影响因子：
--
作者：
Maasch, Christian;Buchner, Klaus;Klussmann, Sven
通讯作者：
Klussmann, Sven