Live imaging of stem cell and progeny behaviour in physiological hair-follicle regeneration.
Live imaging of stem cell and progeny behaviour in physiological hair-follicle regeneration.
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DOI：
10.1038/nature11218
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发表时间：
2012-07-26
期刊：
影响因子：
64.8
通讯作者：
Greco, Valentina
中科院分区：
文献类型：
--
作者：
Rompolas, Panteleimon;Deschene, Elizabeth R.;Zito, Giovanni;Gonzalez, David G.;Saotome, Ichiko;Haberman, Ann M.;Greco, Valentina
组织的发育和再生依赖于细胞间的相互作用和靶向干细胞及其直接后代的信号。然而，导致适当再生组织的细胞行为尚未得到很好的理解。使用新的非侵入性双光子（活体而不是双光子？）成像方法，我们在活体小鼠中真实的时间内研究了生理性毛囊再生。通过这些手段，我们监测了上皮干细胞及其后代在生理毛发再生过程中的行为，并解决了间充质如何影响它们的行为。与早期的研究一致，干细胞在毛发再生的初始阶段是静止的，而后代则更活跃地分裂。此外，干细胞后代分裂在卵泡内空间组织。除了细胞分裂，后代的协调细胞运动允许毛囊的快速扩张。最后，我们通过靶向细胞消融和活毛囊的长期跟踪来显示间充质对毛发再生的要求。因此，我们已经建立了一种体内方法，导致直接观察毛囊内生长调节的细胞机制，并使我们能够精确地研究毛囊成分在生理再生过程中的功能要求。
Tissue development and regeneration depend on cell–cell interactions and signals that target stem cells and their immediate progeny. However, the cellular behaviours that lead to a properly regenerated tissue are not well understood. Using a new, non-invasive, two-photon (intravital instead of two-photon?) imaging approach we study physiological hair-follicle regeneration in real time in live mice. By these means we have monitored the behaviour of epithelial stem cells and their progeny during physiological hair regeneration and addressed how the mesenchyme influences their behaviour. Consistent with earlier studies, stem cells are quiescent during the initial stages of hair regeneration, whereas the progeny is more actively dividing. Moreover, stem cell progeny divisions are spatially organized within follicles. In addition to cell divisions, coordinated cell movements of the progeny allow the rapid expansion of the hair follicle. Finally, we show the requirement of the mesenchyme for hair regeneration through targeted cell ablation and long-term tracking of live hair follicles. Thus, we have established an in vivo approach that has led to the direct observation of cellular mechanisms of growth regulation within the hair follicle and that has enabled us to precisely investigate functional requirements of hair-follicle components during the process of physiological regeneration.
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