Salicylic acid-induced transcriptional reprogramming by the HAC-NPR1-TGA histone acetyltransferase complex in Arabidopsis.
Salicylic acid-induced transcriptional reprogramming by the HAC-NPR1-TGA histone acetyltransferase complex in Arabidopsis.
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DOI：
10.1093/nar/gky847
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发表时间：
2018-12-14
影响因子：
14.9
通讯作者：
Noh B
中科院分区：
文献类型：
--
作者：
Jin H;Choi SM;Kang MJ;Yun SH;Kwon DJ;Noh YS;Noh B
植物免疫依赖于病程相关基因的大量表达，这些基因的转录被病原诱导的信号解除抑制。水杨酸（SA）是植物免疫和系统获得性抗性的主要信号分子。SA信号导致主免疫调节因子，发病相关基因非表达子1（NPR 1）的激活，其被认为被转录因子如TGA募集到许多下游PR。尽管其在SA触发的免疫中的关键作用，NPR 1的转录共激活因子功能的生物化学性质和由其诱导的大规模转录重编程仍然不清楚。在这里，我们证明CBP/p300家族组蛋白乙酰转移酶，HACs和NPR 1都是必不可少的发展SA触发的免疫和PR诱导。事实上，HACs和NPR 1形成共激活因子复合物，并在SA信号后通过TGA募集到PR染色质，最后HAC-NPR 1-TGA复合物通过组蛋白乙酰化介导的表观遗传重编程激活PR转录。因此，我们的研究揭示了NPR 1介导的转录重编程的分子机制和植物中枢免疫系统的关键表观遗传方面。
Plant immunity depends on massive expression of pathogenesis-related genes (PRs) whose transcription is de-repressed by pathogen-induced signals. Salicylic acid (SA) acts as a major signaling molecule in plant immunity and systemic acquired resistance triggered by bacterial or viral pathogens. SA signal results in the activation of the master immune regulator, Nonexpressor of pathogenesis-related genes 1 (NPR1), which is thought to be recruited by transcription factors such as TGAs to numerous downstream PRs. Despite its key role in SA-triggered immunity, the biochemical nature of the transcriptional coactivator function of NPR1 and the massive transcriptional reprogramming induced by it remain obscure. Here we demonstrate that the CBP/p300-family histone acetyltransferases, HACs and NPR1 are both essential to develop SA-triggered immunity and PR induction. Indeed HACs and NPR1 form a coactivator complex and are recruited to PR chromatin through TGAs upon SA signal, and finally the HAC−NPR1−TGA complex activates PR transcription by histone acetylation-mediated epigenetic reprogramming. Thus, our study reveals a molecular mechanism of NPR1-mediated transcriptional reprogramming and a key epigenetic aspect of the central immune system in plants.
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影响因子：
30.8
作者：
Bereshchenko, OR;Gu, W;Dalla-Favera, R
通讯作者：
Dalla-Favera, R
影响因子：
7.2
作者：
Han, Soon-Ki;Song, Ju-Dong;Noh, Bosl
通讯作者：
Noh, Bosl
影响因子：
11.6
作者：
CAO, H;BOWLING, SA;DONG, XN
通讯作者：
DONG, XN
影响因子：
64.8
作者：
Bannister, AJ;Kouzarides, T
通讯作者：
Kouzarides, T
DOI：
10.1073/pnas.92.14.6602
发表时间：
1995-07-03
影响因子：
11.1
作者：
DELANEY, TP;FRIEDRICH, L;RYALS, JA
通讯作者：
RYALS, JA