Optimizing Laguerre expansion based deconvolution methods for analysing bi-exponential fluorescence lifetime images.
Optimizing Laguerre expansion based deconvolution methods for analysing bi-exponential fluorescence lifetime images.
复制标题
优化基于拉盖尔展开的反卷积方法，用于分析双指数荧光寿命图像。
DOI：
10.1364/oe.24.013894
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发表时间：
2016
期刊：
影响因子：
3.8
通讯作者：
D. Li
中科院分区：
文献类型：
--
作者：
Yongliang Zhang;Yu Chen;D. Li
快速去卷积是大荧光寿命成像显微镜（FLIM）图像分析中仪器响应标定的重要步骤。本文研究了一种基于Laguerre展开的计算有效的最小二乘反卷积方法（LSD-LE），最近开发的临床诊断应用，并提出了新的标准选择Laguerre基函数（LBF），而不考虑LBF之间的相互正交性。与先前报道的LSD-LE相比，改进的LSD-LE允许使用更高的激光重复率，减少每次测量的采集时间。此外，我们扩展了它，第一次，分析双指数荧光衰减更一般的FLIM-FRET应用。所提出的方法进行了测试合成的双指数和现实的FLIM数据研究金纳米棒在Hek 293细胞的内吞作用。与以前报道的约束LSD-LE相比，它显示出有前途的结果。
Fast deconvolution is an essential step to calibrate instrument responses in big fluorescence lifetime imaging microscopy (FLIM) image analysis. This paper examined a computationally effective least squares deconvolution method based on Laguerre expansion (LSD-LE), recently developed for clinical diagnosis applications, and proposed new criteria for selecting Laguerre basis functions (LBFs) without considering the mutual orthonormalities between LBFs. Compared with the previously reported LSD-LE, the improved LSD-LE allows to use a higher laser repetition rate, reducing the acquisition time per measurement. Moreover, we extended it, for the first time, to analyze bi-exponential fluorescence decays for more general FLIM-FRET applications. The proposed method was tested on both synthesized bi-exponential and realistic FLIM data for studying the endocytosis of gold nanorods in Hek293 cells. Compared with the previously reported constrained LSD-LE, it shows promising results.
影响因子：
3.6
作者：
Li, David Day-Uei;Yu, Hongqi;Chen, Yu
通讯作者：
Chen, Yu
影响因子：
3.8
作者：
Poland, Simon P.;Erdogan, Ahmet T.;Henderson, Robert K.
通讯作者：
Henderson, Robert K.
影响因子：
62.1
作者：
Berezin, Mikhail Y.;Achilefu, Samuel
通讯作者：
Achilefu, Samuel