The effect of dietary tryptophan levels on oxidative stress of liver induced by diquat in weaned piglets.
The effect of dietary tryptophan levels on oxidative stress of liver induced by diquat in weaned piglets.
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DOI：
10.1186/2049-1891-5-49
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发表时间：
2014
影响因子：
7
通讯作者：
Chen D
中科院分区：
文献类型：
--
作者：
Mao X;Lv M;Yu B;He J;Zheng P;Yu J;Wang Q;Chen D
氧化应激可引起色氨酸代谢异常。本试验旨在研究饲粮色氨酸水平对diquat攻毒断奶仔猪肝脏氧化应激的影响。选取36头21日龄PIC断奶仔猪，随机分为3种饲粮，分别饲喂色氨酸水平为0.18、0.30和0.45%的饲粮，连续饲喂14 d。第8天，仔猪腹腔注射0.9% NaCl无菌溶液或diquat （10 mg/kg体重）。试验前7 d，提高饲粮色氨酸水平提高了仔猪平均日增重（P = 0.09）和平均日采食量（P = 0.08），降低了饲料效率（P < 0.05）。注射双奎特显著降低了猪的生长性能（P < 0.05）。Diquat还降低了仔猪血浆和肝脏超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）活性（P < 0.05），提高了血浆丙二醛（MDA）和尿素氮（P < 0.05）浓度，提高了肝脏MDA浓度（P = 0.09）和色氨酸2,3-双加氧酶（TDO）活性（P = 0.07）。提高饲粮色氨酸水平可以减弱双奎特注射液对仔猪肝脏MDA （P = 0.06）浓度、SOD （P = 0.09）和GPx （P = 0.05）活性以及血浆尿素氮（P = 0.06）浓度的影响。饲粮色氨酸水平与双奎特注射液对提高肝脏TDO活性有协同作用（P < 0.05）。由此可见，提高饲粮色氨酸水平可以通过增强抗氧化能力来减轻腹腔注射迪奎特断奶仔猪肝脏的氧化应激。
Oxidative stress can induce abnormal tryptophan metabolism. The present study was mainly conducted to determine the effect of dietary tryptophan levels on oxidative stress in the liver of weaned pigs challenged by diquat. A total of 36 PIC piglets weaned at 21 days of age were randomly allotted to 1 of 3 diets containing dietary tryptophan levels of 0.18, 0.30, and 0.45% for 14 d. On day 8, the piglets were injected intraperitoneally with sterile 0.9% NaCl solution or diquat (10 mg/kg body weight). During the first 7 d of trial, increasing dietary tryptophan levels enhanced average daily gain (P = 0.09) and average daily feed intake (P = 0.08), and decreased the feed efficiency (P < 0.05) of piglets. The growth performance was decreased by diquat injection (P < 0.05). Diquat injection also decreased the activities of the superoxide dismutase (SOD) and glutathione peroxidase (GPx) in the plasma and liver (P < 0.05), increased plasma malondialdehyde (MDA) (P < 0.05) and urea nitrogen (P < 0.05) concentrations, and enhanced MDA concentration (P = 0.09) and tryptophan 2,3-dioxygenase (TDO) activity (P = 0.07) in liver of piglets. Increasing dietary tryptophan levels could attenuate the effects of diquat injection on the MDA (P = 0.06) concentration and the activities of SOD (P = 0.09) and GPx (P = 0.05) of the liver, and plasma urea nitrogen (P = 0.06) concentration in the piglet. There was a synergistic role for increasing TDO activity in the liver between dietary tryptophan levels and diquat injection (P < 0.05). These results suggest that increasing dietary tryptophan levels could attenuate the oxidative stress of the liver in weaned piglets intraperitoneally injected with diquat via enhancing the antioxidant capacity.
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影响因子：
3.3
作者：
Koopmans, SJ;Guzik, AC;Southern, LL
通讯作者：
Southern, LL
DOI：
10.1016/j.pathophys.2006.05.007
发表时间：
2006-08-01
期刊：
Pathophysiology : the official journal of the International Society for Pathophysiology
影响因子：
--
作者：
Chauhan, Abha;Chauhan, Ved
通讯作者：
Chauhan, Ved
影响因子：
6.1
作者：
Dairam, A.;Antunes, E. M.;Daya, S.
通讯作者：
Daya, S.
影响因子：
1.8
作者：
Mao, Xiangbing;Qi, Sharina;Chen, Daiwen
通讯作者：
Chen, Daiwen
影响因子：
7
作者：
LEATHWOOD, PD
通讯作者：
LEATHWOOD, PD