The inhibition of Staphylococcus epidermidis biofilm formation by vancomycin-modified titanium alloy and implications for the treatment of periprosthetic infection.
The inhibition of Staphylococcus epidermidis biofilm formation by vancomycin-modified titanium alloy and implications for the treatment of periprosthetic infection.
复制标题
DOI：
10.1016/j.biomaterials.2008.08.016
复制
发表时间：
2008-12
期刊：
影响因子：
14
通讯作者：
Hickok, Noreen J.
中科院分区：
文献类型：
--
作者：
Antoci, Valentin, Jr.;Adams, Christopher S.;Parvizi, Javad;Davidson, Helen M.;Composto, Russell J.;Freeman, Theresa A.;Wickstrom, Eric;Ducheyne, Paul;Jungkind, Donald;Shapiro, Irving M.;Hickok, Noreen J.
关键词：
众所周知，假体周围感染很难治疗，因为生物材料植入物是细菌粘附和生物膜形成的理想选择，导致抗生素敏感性降低。先前，我们报道了万古霉素共价附着在钛合金表面（van -Ti）可以阻止细菌定植。在这里，我们研究了这种van - ti表面对表皮葡萄球菌的影响，表皮葡萄球菌是一种在骨科感染中普遍存在的革兰氏阳性菌。通过对活菌的直接菌落计数和荧光显示，表皮葡萄球菌在van - ti植入物上的定植被显著抑制。相比之下，革兰氏阴性菌大肠杆菌很容易在van - ti棒上定植，这表明保留了抗生素的特异性。通过组织化学和扫描电镜分析，即使在血清存在的情况下，van - ti也能阻止表皮葡萄球菌生物膜的形成。此外，当被表皮葡萄球菌多次攻击时，van - ti棒能够抵抗细菌的定植。最后，当表皮葡萄球菌在有van - ti存在的情况下持续培养时，该细菌保持了与亲本菌株相当的Vanc敏感性。这些发现表明，植入物的抗生素衍生化可以产生可以抵抗细菌定植的表面。这项技术为预防和治疗假体周围感染带来了巨大的希望。
Peri-prosthetic infections are notoriously difficult to treat as the biomaterial implant is ideal for bacterial adhesion and biofilm formation, resulting in decreased antibiotic sensitivity. Previously, we reported that vancomycin covalently attached to a Ti alloy surface (Vanc-Ti) could prevent bacterial colonization. Herein we examine the effect of this Vanc-Ti surface on Staphylococci epidermidis, a Gram-positive organism prevalent in orthopaedic infections. By direct colony counting and fluorescent visualization of live bacteria, S. epidermidis colonization was significantly inhibited on Vanc-Ti implants. In contrast, the gram negative organism Escherichia coli readily colonized the Vanc-Ti rod, suggesting retention of antibiotic specificity. By histochemical and SEM analysis, Vanc-Ti prevented S. epidermidis biofilm formation, even in the presence of serum. Furthermore, when challenged multiple times with S. epidermidis, Vanc-Ti rods resisted bacterial colonization. Finally, when S. epidermidis was continuously cultured in the presence of Vanc-Ti, the bacteria maintained a Vanc sensitivity equivalent to the parent strain. These findings indicate that antibiotic derivatization of implants can result in a surface that can resist bacterial colonization. This technology holds great promises for the prevention and treatment of periprosthetic infections.
登录
查看更多内容
DOI：
10.1302/0301-620x.88b2.17058
发表时间：
2006-02-01
影响因子：
--
作者：
Toms, AD;Davidson, D;Duncan, CP
通讯作者：
Duncan, CP
DOI：
10.1097/00003086-200111000-00003
发表时间：
2001-11-01
影响因子：
4.2
作者：
Peersman, G;Laskin, R;Peterson, M
通讯作者：
Peterson, M
影响因子：
14
作者：
BARTH, E;MYRVIK, QM;GRISTINA, AG
通讯作者：
GRISTINA, AG
影响因子：
3.3
作者：
Oliveira, M.;Bexiga, R.;Vilela, C. L.
通讯作者：
Vilela, C. L.
影响因子：
4.6
作者：
Vodna, Lucia;Bubenikova, Silvia;Bakos, Dusan
通讯作者：
Bakos, Dusan