Contrasting quiescent G0 phase with mitotic cell cycling in the mouse immune system.

Contrasting quiescent G0 phase with mitotic cell cycling in the mouse immune system.
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将静态G0相与小鼠免疫系统中的有丝分裂细胞循环对比。

DOI:
10.1371/journal.pone.0073801
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发表时间:
2013
期刊:
影响因子:
3.7
通讯作者:
Miyawaki A
Miyawaki A
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Tomura M;Sakaue-Sawano A;Mori Y;Takase-Utsugi M;Hata A;Ohtawa K;Kanagawa O;Miyawaki A

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表达Fucci(荧光泛素化细胞周期指示剂)探针的转基因小鼠系使我们能够监测造血系统中的细胞周期。通过FACS分析鉴定了两个具有高和低强度的Cdt1(30/120)积累的Fucci信号的群体,并且这些分别对应于静止G0和循环G1细胞。我们观察了淋巴器官中的免疫细胞在分化和免疫应答过程中在静止期和增殖期之间的转变。
A transgenic mouse line expressing Fucci (fluorescent ubiquitination-based cell-cycle indicator) probes allows us to monitor the cell cycle in the hematopoietic system. Two populations with high and low intensities of Fucci signals for Cdt1(30/120) accumulation were identified by FACS analysis, and these correspond to quiescent G0 and cycling G1 cells, respectively. We observed the transition of immune cells between quiescent and proliferative phases in lymphoid organs during differentiation and immune responses.
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