Myosin II contributes to cell-scale actin network treadmilling through network disassembly.
Myosin II contributes to cell-scale actin network treadmilling through network disassembly.
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作者：
Crawling locomotion of eukaryotic cells is achieved by a process dependent on the actin cytoskeleton: protrusion of the leading edge requires assembly of a network of actin filaments, which must be disassembled at the cell rear for sustained motility. Although ADF/cofilin proteins have been shown to contribute to actin disassembly, it is not clear how activity of these locally acting proteins could be coordinated over the whole-cell distance scale. Here we show that nonmuscle myosin II plays a direct role in actin network disassembly in crawling cells. In moving fish keratocytes, myosin II is concentrated in regions at the rear with high rates of network disassembly. Activation of myosin II by ATP in detergent-extracted cytoskeletons results in rear-localized disassembly of the actin network. Inhibition of myosin II activity and stabilization of actin filaments synergistically impede cell motility, suggesting the existence of two disassembly pathways, one of which requires myosin II activity. Our results establish the importance of myosin II as an enzyme for actin network disassembly; we propose that gradual formation and reorganization of an actomyosin network provides an intrinsic destruction timer, enabling long-range coordination of actin network treadmilling in motile cells.
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影响因子：
56.9
作者：
Straight, AF;Cheung, A;Mitchison, TJ
通讯作者：
Mitchison, TJ
DOI：
10.1016/j.bbrc.2004.06.045
发表时间：
2004-07-30
影响因子：
3.1
作者：
Kolega, J
通讯作者：
Kolega, J
影响因子：
5.6
作者：
Haviv, Lior;Gillo, David;Bernheim-Groswasser, Anne
通讯作者：
Bernheim-Groswasser, Anne
DOI：
10.1073/pnas.0300552101
发表时间：
2004-06-29
影响因子：
11.1
作者：
Vallotton, P;Gupton, SL;Danuser, G
通讯作者：
Danuser, G
影响因子：
9.2
作者：
Sai, XiaoRei;Ladher, Raj K.
通讯作者：
Ladher, Raj K.