Phytophthora infestans RXLR effector SFI5 requires association with calmodulin for PTI/MTI suppressing activity.
Phytophthora infestans RXLR effector SFI5 requires association with calmodulin for PTI/MTI suppressing activity.
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致病疫霉 RXLR 效应子 SFI5 需要与钙调素结合才能抑制 PTI/MTI 活性
DOI：
10.1111/nph.15250
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发表时间：
2018-09
期刊：
影响因子：
--
通讯作者：
Brunner F
中科院分区：
文献类型：
--
作者：
Zheng X;Wagener N;McLellan H;Boevink PC;Hua C;Birch PRJ;Brunner F
病原菌通过分泌效应蛋白来干扰植物的天然免疫，其中Ca 2 +/钙调素（CaM）信号转导起着关键作用。到目前为止，很少有效应物被确定为直接与钙调素相互作用的防御抑制。在这里，我们报告了SFI 5，一种来自致病疫霉的RXLR效应子，通过与宿主CaMs相互作用来抑制微生物相关分子模式（MAMP）触发的免疫（MTI）。我们使用计算机模拟分析预测了SFI 5中的CaM结合位点。SFI 5和CaM之间的相互作用通过体外和体内测定来测试。在番茄原生质体系统中进行了SFI 5和截短变体对MTI的抑制。我们发现预测的CaM结合位点和全长SFI 5蛋白在Ca 2+存在下与CaM相互作用。MTI反应，如FRK 1上调，活性氧积累，和丝裂原活化蛋白激酶激活被截短的SFI 5蛋白抑制含有C末端CaM结合位点，但不被那些没有它。质膜定位的SFI 5和它的能力，以增强感染也受到干扰CaM结合位点的损失。我们得出结论，CaM结合是SFI 5定位和活性所必需的。我们认为SFI 5通过干扰钙调素激活后的免疫信号传导成分来抑制植物免疫。
Pathogens secrete effector proteins to interfere with plant innate immunity, in which Ca2+/calmodulin (CaM) signalling plays key roles. Thus far, few effectors have been identified that directly interact with CaM for defence suppression. Here, we report that SFI5, an RXLR effector from Phytophthora infestans, suppresses microbe‐associated molecular pattern (MAMP)‐triggered immunity (MTI) by interacting with host CaMs. We predicted the CaM‐binding site in SFI5 using in silico analysis. The interaction between SFI5 and CaM was tested by both in vitro and in vivo assays. MTI suppression by SFI5 and truncated variants were performed in a tomato protoplast system. We found that both the predicted CaM‐binding site and the full‐length SFI5 protein interact with CaM in the presence of Ca2+. MTI responses, such as FRK1 upregulation, reactive oxygen species accumulation, and mitogen‐activated protein kinase activation were suppressed by truncated SFI5 proteins containing the C‐terminal CaM‐binding site but not by those without it. The plasma membrane localization of SFI5 and its ability to enhance infection were also perturbed by loss of the CaM‐binding site. We conclude that CaM‐binding is required for localization and activity of SFI5. We propose that SFI5 suppresses plant immunity by interfering with immune signalling components after activation by CaMs.
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用一块石头杀死两只鸟：T3E从肠病细菌中抑制PAMP/MAMP诱导的免疫力。
影响因子：
5.2
作者：
Fraiture M;Brunner F
通讯作者：
Brunner F
影响因子：
12.3
作者：
Day IS;Reddy VS;Shad Ali G;Reddy AS
通讯作者：
Reddy AS
DOI：
10.1007/978-1-62703-986-4_17
发表时间：
2014-01-01
期刊：
PLANT-PATHOGEN INTERACTIONS: METHODS AND PROTOCOLS, 2ND EDITION
影响因子：
--
作者：
Fraiture, Malou;Zheng, Xiangzi;Brunner, Frederic
通讯作者：
Brunner, Frederic
DOI：
10.1007/978-1-61779-821-4_5
发表时间：
2012-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Arndt, Claudia;Koristka, Stefanie;Bachmann, Michael
通讯作者：
Bachmann, Michael
影响因子：
16.6
作者：
Boevink PC;Wang X;McLellan H;He Q;Naqvi S;Armstrong MR;Zhang W;Hein I;Gilroy EM;Tian Z;Birch PRJ
通讯作者：
Birch PRJ