Qualitative analysis of algal secretions with multiple mass spectrometric platforms.
Qualitative analysis of algal secretions with multiple mass spectrometric platforms.
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DOI：
10.1016/j.chroma.2012.04.074
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发表时间：
2012-06-29
期刊：
影响因子：
--
通讯作者：
Fiehn O
中科院分区：
文献类型：
--
作者：
Kind T;Meissen JK;Yang D;Nocito F;Vaniya A;Cheng YS;Vandergheynst JS;Fiehn O
来自藻类的脂质分泌物为工程生物燃料原料提供了巨大的机会。从工艺工程的角度来看，脂质渗出物可能是令人感兴趣的，因为脂质可以直接从培养基中收集，而无需收获和破坏细胞。我们在这里报告的藻类代谢产物的细胞外分泌物从菌株UTEX 2341（小球藻）到培养基中。迄今为止，尚未对这些脂质分泌物进行详细分析。使用多个质谱平台，我们观察到约1000种化合物，并能够通过液相色谱法结合精确的质量四极杆飞行时间质谱法（LC-QTOF），直接输注正负电喷雾离子阱质谱法和气相色谱法结合质谱法（GC-MS）注释50种脂质。这些化合物通过串联质谱（MS/MS）数据库匹配和保留时间范围过滤进行注释。我们观察到一系列的三酰甘油（TG），磺基quinovosyldiacylglycerols（SQDG），磷脂酰肌醇和磷脂酰甘油，以及甜菜碱脂质二酰甘油-N，N，N-三甲基高丝氨酸（DGTS）。
Lipid secretions from algae pose a great opportunity for engineering biofueler feedstocks. The lipid exudates could be interesting from a process engineering perspective because lipids could be collected directly from the medium without harvesting and disrupting cells. We here report on the extracellular secretions of algal metabolites from the strain UTEX 2341 (Chlorella minutissima) into the culture medium. No detailed analysis of these lipid secretions has been performed to date. Using multiple mass spectrometric platforms, we observed around 1000 compounds and were able to annotate 50 lipids by means of liquid chromatography coupled to accurate mass quadrupole time-of-flight mass spectrometry (LC-QTOF), direct infusion with positive and negative electrospray ion trap mass spectrometry and gas chromatography coupled to mass spectrometry (GC–MS). These compounds were annotated by tandem mass spectral (MS/MS) database matching and retention time range filtering. We observed a series of triacylglycerols (TG), sulfoquinovosyldiacylglycerols (SQDG), phosphatidylinositols and phosphatidylglycerols, as well as betaine lipids diacylglyceryl-N,N,N-trimethylhomoserines (DGTS).
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影响因子：
7.4
作者：
Cody, RB;Laramée, JA;Durst, HD
通讯作者：
Durst, HD
影响因子：
7.4
作者：
CHANG, WH;TOLBERT, NE
通讯作者：
TOLBERT, NE
影响因子：
1.6
作者：
HALLEGRAEFF, GM
通讯作者：
HALLEGRAEFF, GM
影响因子：
3.5
作者：
Barupal DK;Kind T;Kothari SL;Lee DY;Fiehn O
通讯作者：
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影响因子：
12.3
作者：
Herzog R;Schwudke D;Schuhmann K;Sampaio JL;Bornstein SR;Schroeder M;Shevchenko A
通讯作者：
Shevchenko A