Temporal and spatial expression of AIF in the mouse uterus during early pregnancy.
Temporal and spatial expression of AIF in the mouse uterus during early pregnancy.
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妊娠早期小鼠子宫中 AIF 的时空表达。
DOI：
10.2741/450
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
单春华
中科院分区：
文献类型：
--
作者：
张丽英;华育平;安铁洙;聂卫平;王连邦;单春华
凋亡诱导因子（Apoptosis-inducing factor，AIF）是一种具有促凋亡功能和氧化还原活性的双功能蛋白质。AIF通过其亚细胞定位调节细胞凋亡并在抗应激中发挥作用。胚胎植入是一个复杂的过程，其中激活的囊胚与接受性子宫相互作用。本研究采用原位杂交、免疫组织化学和实时荧光定量PCR技术，研究了AIF在小鼠早孕、假孕、延迟着床、人工蜕膜化和激素处理子宫中的表达和调控。在妊娠早期，AIF在小鼠子宫中的表达存在时空调控模式; AIF在子宫腔上皮和腺上皮中的表达受类固醇激素的调节; AIF mRNA在间质中的表达受活跃囊胚的影响;共聚焦显微镜发现AIF蛋白定位于细胞质而非细胞核。我们的数据表明，AIF可能在小鼠胚胎着床过程中发挥重要作用，AIF的作用可能是通过其生理活性，而不是通过其促凋亡功能在小鼠子宫在此期间实施。
Apoptosis-inducing factor (AIF) is a phylogenetically old, bifunctional protein with a pro-apoptotic function and redox activity. AIF regulates apoptosis and also plays a role in the defense against stress depending on its subcellular localization. Embryo implantation is a complicated process, in which an activated blastocyst interacts with a receptive uterus. The expression and regulation of AIF were investigated in this study in the mouse uterus during early pregnancy, pseudopregnancy, delayed implantation, artificial decidualization and under hormonal treatment using in situ hybridization, immunohistochemistry and real-time PCR. During early pregnancy, temporally and spatially regulated patterns of AIF expression were found in the mouse uterus; AIF expression in the luminal epithelium and glandular epithelium is regulated by steroid hormones; AIF mRNA expression in the stroma is influenced by the active blastocyst; and AIF protein was found to be located in the cytoplasm rather than the nucleus through confocal microscope. Our data suggest that AIF might play an important role during mouse embryo implantation and that the role of AIF might be implemented through its physiological activity rather than through its pro-apoptotic function in the mouse uterus during this period.
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DOI：
10.1530/jrf.0.0840539
发表时间：
1988-11
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
H. Weitlauf;M. Suda-Hartman
通讯作者：
H. Weitlauf;M. Suda-Hartman
影响因子：
2.9
作者：
Li, Guang-Yu;Osborne, Neville N.
通讯作者：
Osborne, Neville N.
影响因子：
8.8
作者：
Yang, X.;Fraser, M.;Abedini, M. R.;Bai, T.;Tsang, B. K.
通讯作者：
Tsang, B. K.
DOI：
--
发表时间：
2005
期刊：
--
影响因子：
--
作者：
Carl Shooner;Pierre-Luc Caron;Guylaine Fréchette-Frigon;Valérie Leblanc;Marie-Claude Déry;E. Asselin
通讯作者：
Carl Shooner;Pierre-Luc Caron;Guylaine Fréchette-Frigon;Valérie Leblanc;Marie-Claude Déry;E. Asselin
影响因子：
64.8
作者：
Susin, SA;Lorenzo, HK;Kroemer, G
通讯作者：
Kroemer, G