Transient and Partial Nuclear Lamina Disruption Promotes Chromosome Movement in Early Meiotic Prophase.
Transient and Partial Nuclear Lamina Disruption Promotes Chromosome Movement in Early Meiotic Prophase.
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DOI：
10.1016/j.devcel.2018.03.018
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发表时间：
2018-04-23
影响因子：
11.8
通讯作者：
Jantsch V
中科院分区：
文献类型：
--
作者：
Link J;Paouneskou D;Velkova M;Daryabeigi A;Laos T;Labella S;Barroso C;Pacheco Piñol S;Montoya A;Kramer H;Woglar A;Baudrimont A;Markert SM;Stigloher C;Martinez-Perez E;Dammermann A;Alsheimer M;Zetka M;Jantsch V
减数分裂染色体的运动对同源染色体的成对排列是重要的，这是正确的染色体分离所必需的。运动由细胞质力驱动，通过核膜跨越蛋白传递到染色体末端。在动物细胞中，层粘连蛋白在核周围形成一个突出的支架，但层粘连蛋白在减数分裂染色体运动中的作用尚不清楚。我们发现染色体运动与核边缘层状蛋白关联的减少相关，这需要层状蛋白在类似于有丝分裂中打开层状网络交联的位点磷酸化。膜重塑失败导致减数分裂进入延迟，染色质组织改变，染色体不配对或连锁，染色体运动减慢。重塑激酶通过与核膜相连的染色体末端传递给层状蛋白，从而潜在地实现了层状蛋白和染色体事件之间的串扰。因此，在减数分裂期间，打开层状网络在调节染色体和核外周之间的接触中起作用。在进入减数分裂后，核板的蛋白质网络被打开/松散，核板交联的减弱引发了染色质的重组，而核板交联的重塑失败会导致染色体形态的异常。减数分裂前期核板的磷酸化是维持染色体完整性的必要条件。Link、Paouneskou等人的研究表明，减数分裂层交联重构通过连接到核膜的染色体末端传递的激酶磷酸化，确保了染色体的完整性。
Meiotic chromosome movement is important for the pairwise alignment of homologous chromosomes, which is required for correct chromosome segregation. Movement is driven by cytoplasmic forces, transmitted to chromosome ends by nuclear membrane-spanning proteins. In animal cells, lamins form a prominent scaffold at the nuclear periphery, yet the role lamins play in meiotic chromosome movement is unclear. We show that chromosome movement correlates with reduced lamin association with the nuclear rim, which requires lamin phosphorylation at sites analogous to those that open lamina network crosslinks in mitosis. Failure to remodel the lamina results in delayed meiotic entry, altered chromatin organization, unpaired or interlocked chromosomes, and slowed chromosome movement. The remodeling kinases are delivered to lamins via chromosome ends coupled to the nuclear envelope, potentially enabling crosstalk between the lamina and chromosomal events. Thus, opening the lamina network plays a role in modulating contacts between chromosomes and the nuclear periphery during meiosis. Upon meiotic entry, the protein network of the nuclear lamina is opened up/loosened Weakening of lamin crosslinks triggers dramatic chromatin reorganization Failure to remodel lamina crosslinking can lead to aberrant chromosome forms Meiotic phosphorylation of lamin is essential for maintaining chromosome integrity The onset of chromosome movement within meiotic prophase nuclei is accompanied by lamina structural changes resembling those associated with mitotic nuclear envelope breakdown. Link, Paouneskou et al. show that meiotic lamina crosslink remodeling, through phosphorylation by kinases delivered via chromosome ends coupled to the nuclear envelope, ensures chromosome integrity.
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影响因子：
4.5
作者：
Link J;Jahn D;Schmitt J;Göb E;Baar J;Ortega S;Benavente R;Alsheimer M
通讯作者：
Alsheimer M
影响因子：
3.3
作者：
Jantsch, Verena;Tang, Lois;Loidl, Josef
通讯作者：
Loidl, Josef
影响因子：
10.5
作者：
Martinez-Perez, E;Villeneuve, AM
通讯作者：
Villeneuve, AM
影响因子：
11.8
作者：
Colaiácovo, MP;MacQueen, AJ;Villeneuve, AM
通讯作者：
Villeneuve, AM
影响因子：
16
作者：
Gartner, A;Milstein, S;Hengartner, MO
通讯作者：
Hengartner, MO