Toxicity-efficacy converting of ginseng combined with Fuzi Banxia incompatibility in heart failure stage of cor pulmonale
Toxicity-efficacy converting of ginseng combined with Fuzi Banxia incompatibility in heart failure stage of cor pulmonale
复制标题
人参与附子半夏配伍对肺心病心力衰竭期的毒效转换
DOI：
10.1016/j.chmed.2018.03.010
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发表时间：
2018-04
影响因子：
3.8
通讯作者：
Yan-jun Zhang
中科院分区：
文献类型：
--
作者：
Ying-ying Huang;Xiao-jin Li;Kai-xin Liu;Peng-wei Zhuang;Yan-jun Zhang
目的附子半夏为十八拮之一，以往的研究表明，在特定的病况和适宜的配伍环境下，配伍禁忌可以发挥特殊的功效。本研究旨在评价人参联合附子半夏配伍干预肺心病心力衰竭阶段的毒效并探讨其作用机制。方法本研究采用野百合碱(MCT)诱导肺心病。采用超高分辨率小动物超声实时成像系统和右心导管检查评估心功能。采用半自动生化分析仪检测血清中心肌酶LDH、CK、CK-MB。心脏组织HE染色，TUNEL法评估病理形态学变化及心肌细胞凋亡。 RT-PCR检测右心室肥厚及凋亡相关基因ANP、BNP、β-MHC、Bax、Bcl-2的表达。结果附子半夏联合人参明显降低死亡率，降低RVHI，升高心脏指数； RVSP和mPAP明显降低，EF和FS明显升高；心肌酶 LDH、CK 和 CK-MB 明显减弱；心脏直径缩小，右心室扩张明显减少，炎症细胞浸润明显减少，心肌细胞凋亡率明显降低。同时肥大相关的ANP、BNP、β-MHC mRNA表达上调，凋亡相关Bax mRNA表达下调，抗凋亡相关Bcl-2 mRNA表达及Bcl-2/Bax比值上调。结论人参配伍环境可减轻附子半夏不配伍干预肺心病心力衰竭阶段的心脏毒性，改善心脏功能功能，可能与肥厚和凋亡相关基因的表达有关，从而延缓心力衰竭的发生和发展。
ObjectiveFuzi Banxia is one of eighteen antagonisms, previous studies have shown that the incompatibility could play special effects in the specific condition of diseases and appropriate compatible environment. The present study aims to evaluate the toxicity-efficacy of ginseng combined with Fuzi Banxia incompatibility intervening in the heart failure stage of cor pulmonale and to explore its mechanism.MethodsMonocrotaline (MCT)-induced cor pulmonale were used in this study. Ultra high-resolution small animal ultrasound real-time imaging system and the right heart catheterization were used to estimate cardiac function. Semi automatic biochemical analyzer was used to test myocardial enzyme LDH, CK, and CK-MB in serum. The heart tissues were stained with HE, and TUNEL assay was used to assess the pathomorphological changes and myocardial apoptosis. The expression of hypertrophy and apoptosis associated genes: ANP, BNP, β-MHC, Bax, and Bcl-2 in the right ventricle were determined by RT-PCR.ResultsFuzi Banxia combined with ginseng obviously attenuated mortality, decreased RVHI, and increased cardiac index; RVSP and mPAP were significantly reduced, and EF and FS were raised obviously; Myocardial enzymes LDH, CK, and CK-MB were pronounced attenuated; heart diameter reduced, right ventricular dilatation was significantly decreased, inflammatory cell infiltration notably reduced, and cardiac apoptosis rate was decreased obviously. Meanwhile, the expression of hypertrophy-related ANP, BNP, and β-MHC mRNA were up-regulated, the expression of apoptosis-related Bax mRNA was down-regulated, and the expression of anti-apoptosis-related Bcl-2 mRNA and Bcl-2/Bax ratio were up-regulated.ConclusionGinseng compatible environment could attenuate cardiac toxicity of Fuzi Banxia incompatibility intervening in the heart failure stage of cor pulmonale, and improve cardiac function, which may be related to the expression of hypertrophy and apoptosis associated genes, and thus delay the occurrence and development of heart failure.
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