Disrupted diurnal oscillations of the gut microbiota in patients with alcohol dependence.
Disrupted diurnal oscillations of the gut microbiota in patients with alcohol dependence.
复制标题
酒精依赖患者肠道微生物群的昼夜振荡被破坏。
DOI：
10.3389/fcimb.2023.1127011
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发表时间：
2023
影响因子：
5.7
通讯作者：
中科院分区：
文献类型：
--
作者：
酒精依赖（AD）患者可表现出肠道菌群失调。菌群失调可能与肠道植物群的昼夜节律紊乱同时发生，这可能加重AD。在此，本研究旨在研究AD患者肠道菌群的昼夜振荡。根据精神疾病诊断和统计手册第4版，32例AD患者和20例健康受试者入组本研究。通过自我报告问卷收集人口统计学和临床数据。在7：00 AM、11：00 AM、3：00 PM和7：00 PM采集每例受试者的粪便样本。进行16 S rDNA测序。进行Wilcoxon和Kruskal-Wallis检验以表征肠道微生物群的改变和振荡。我们发现，与健康受试者相比，AD患者肠道微生物群的β多样性每日振荡（p = 0.01）。此外，0.66%的操作分类单位昼夜振荡AD患者与1.68%的健康受试者。在不同的分类水平，细菌丰度在两组中，如假单胞菌和普氏菌pallens的日振荡（所有p < 0.05）。与其他AD患者相比，每日饮酒量高、高水平渴望、AD持续时间短和轻度戒断症状的AD患者的肠道微生物群的β多样性每日振荡（所有p < 0.05）。AD患者的肠道菌群表现出昼夜振荡的破坏，这可能为AD的机制和治疗策略的发展提供新的见解。
Patients with alcohol dependence (AD) can exhibit gut dysbacteria. Dysbacteria may co-occur with disruptions of circadian rhythmicity of the gut flora, which can aggravate AD. Herein, this study aimed to investigate diurnal oscillations of the gut microbiota in AD patients. Thirty-two patients with AD, based on the Diagnostic and Statistical Manual of Mental Disorders, 4th edition, and 20 healthy subjects were enrolled in this study. Demographic and clinical data were collected by self-report questionnaires. Fecal samples at 7:00 AM, 11:00 AM, 3:00 PM, and 7:00 PM were collected from each subject. 16S rDNA sequencing was conducted. Wilcoxon and Kruskal-Wallis tests were performed to characterize alterations and oscillations of the gut microbiota. We found that β-diversity of the gut microbiota in AD patients oscillated diurnally compared with healthy subjects (p = 0.01). Additionally, 0.66% of operational taxonomic units oscillated diurnally in AD patients versus 1.68% in healthy subjects. At different taxonomic levels, bacterial abundance oscillated diurnally in both groups, such as Pseudomonas and Prevotella pallens (all p < 0.05). β-diversity of the gut microbiota in AD patients with high daily alcohol consumption, high-level cravings, short AD durations, and mild withdrawal symptoms oscillated diurnally compared with other AD patients (all p < 0.05). The gut microbiota in AD patients exhibits disruptions of diurnal oscillation, which may provide novel insights into mechanisms of AD and the development of therapeutic strategies.
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影响因子：
15.5
作者：
Dubinkina VB;Tyakht AV;Odintsova VY;Yarygin KS;Kovarsky BA;Pavlenko AV;Ischenko DS;Popenko AS;Alexeev DG;Taraskina AY;Nasyrova RF;Krupitsky EM;Shalikiani NV;Bakulin IG;Shcherbakov PL;Skorodumova LO;Larin AK;Kostryukova ES;Abdulkhakov RA;Abdulkhakov SR;Malanin SY;Ismagilova RK;Grigoryeva TV;Ilina EN;Govorun VM
通讯作者：
Govorun VM
影响因子：
4.7
作者：
Falcón E;McClung CA
通讯作者：
McClung CA
影响因子：
27.4
作者：
Dudek M;Yang N;Ruckshanthi JP;Williams J;Borysiewicz E;Wang P;Adamson A;Li J;Bateman JF;White MR;Boot-Handford RP;Hoyland JA;Meng QJ
通讯作者：
Meng QJ
影响因子：
56.9
作者：
Ley, Ruth E.;Hamady, Micah;Gordon, Jeffrey I.
通讯作者：
Gordon, Jeffrey I.
DOI：
10.1111/j.1530-0277.1999.tb04349.x
发表时间：
1999-08-01
影响因子：
3.2
作者：
Flannery, BA;Volpicelli, JR;Pettinati, HM
通讯作者：
Pettinati, HM