Quantification of thiols and disulfides.
Quantification of thiols and disulfides.
复制标题
DOI：
10.1016/j.bbagen.2013.03.031
复制
发表时间：
2014-02
影响因子：
3
通讯作者：
Thorpe, Colin
中科院分区：
文献类型：
--
作者：
Winther, Jakob R.;Thorpe, Colin
二硫键的形成是一种关键的翻译后修饰，对许多蛋白质的结构、功能和稳定性具有重要意义。虽然二硫键的形成是许多蛋白质的必要和基本过程，但它对其他蛋白质是有害的和破坏性的。细胞竭尽全力调节巯基-二硫键的稳态，通常有几个明显冗余的系统并行工作。剖析二硫化物的氧化和还原程度是一个持续的挑战，部分原因是硫醇/二硫化物交换反应的设施。在本报告中，我们简要地调查了工具箱可用于硫醇和二硫化物的化学测定实验。我们选择专注于当前方法的关键化学方面，同时确定其实验实施中固有的潜在困难。虽然已经描述了许多试剂用于测量和操纵硫醇和二硫化物的氧化还原状态，但这些方法中的许多方法仍然未得到充分利用。有效量化活细胞中氧化还原条件变化的能力是一个持续的挑战。在硫醇和二硫化物的代谢互变中仍存在许多未解决的问题。例如，虽然池大小的氧化还原对和它们的细胞内分布正在被揭露，很少有人知道在硫醇-二硫化物交换途径的流量。需要新的工具来解决细胞代谢的这一重要方面。
Disulfide bond formation is a key posttranslational modification, with implications for structure, function and stability of numerous proteins. While disulfide bond formation is a necessary and essential process for many proteins, it is deleterious and disruptive for others. Cells go to great lengths to regulate thiol-disulfide bond homeostasis, typically with several, apparently redundant, systems working in parallel. Dissecting the extent of oxidation and reduction of disulfides is an ongoing challenge due, in part, to the facility of thiol/disulfide exchange reactions. In the present account, we briefly survey the toolbox available to the experimentalist for the chemical determination of thiols and disulfides. We have chosen to focus on the key chemical aspects of current methodology, together with identifying potential difficulties inherent in their experimental implementation. While many reagents have been described for the measurement and manipulation of the redox status of thiols and disulfides, a number of these methods remain underutilized. The ability to effectively quantify changes in redox conditions in living cells presents a continuing challenge. Many unresolved questions in the metabolic interconversion of thiols and disulfides remain. For example, while pool sizes of redox pairs and their intracellular distribution are being uncovered, very little is known about the flux in thiol-disulfide exchange pathways. New tools are needed to address this important aspect of cellular metabolism.
登录
查看更多内容
影响因子：
1.7
作者：
Bagiyan, GA;Koroleva, IK;Ufimtsev, AV
通讯作者：
Ufimtsev, AV
影响因子：
2.9
作者：
Björnberg, O;Ostergaard, H;Winther, JR
通讯作者：
Winther, JR
影响因子：
3.5
作者：
CHAU, MH;NELSON, JW
通讯作者：
NELSON, JW
DOI：
10.1016/s0731-7085(01)00658-6
发表时间：
2002-05-15
影响因子：
3.4
作者：
Abukhalaf, IK;Silvestrov, NA;Ganafa, AA
通讯作者：
Ganafa, AA
影响因子：
2.9
作者：
GEHRING, H;CHRISTEN, P
通讯作者：
CHRISTEN, P