Spatiotemporal Variability in Microbial Quality of Western US Agricultural Water Supplies: A Multistate Study.
Spatiotemporal Variability in Microbial Quality of Western US Agricultural Water Supplies: A Multistate Study.
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美国西部农业供水微生物质量的时空变异：一项多州研究。
DOI：
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发表时间：
2018
影响因子：
2.4
通讯作者：
E. Atwill
中科院分区：
文献类型：
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作者：
M. Partyka;Ronald F Bond;J. Chase;E. Atwill
2011年，美国国会通过了《食品安全现代化法案》，该法案要求美国食品和药物管理局为农业用水建立微生物标准。然而，关于地表水灌溉用品的微生物质量的数据很少。在2015年灌溉季节，我们对加利福尼亚州(=2)和华盛顿州(4)大型灌区的微生物水质进行了基线研究。对每月样本(517份)进行细菌指标(粪便大肠菌群、肠球菌和)和病原体(Sp.、O157和非O157志贺毒素产生菌[STEC])的分析。虽然有很高的变异性(μ±SD=59.13±106.0)，但只有11%(56/517)的样本超过了126CFU100mL，只有6个样本超过了410CFU100mL。采集了两份水(L 1份和L 10份)进行病原菌分析；10份L样本中的感染率(68149)几乎是1份L样本中发现的两倍。我们在∼期间发现STEC占抽样事件的9%(58/517)；O26和O45型分别为最常见的31%和26%。病原体与超过调节阈值无关，然而，浊度每对数增加，检测到的几率增加约三倍(优势比[OR.]=3.14，0.0001)。微生物学结果具有高度的地区特殊性，表明水质的驱动因素在时空尺度上有所不同。灌溉用水产品受到污染的真正风险以及改善食品安全的最佳监测战略仍然未知。
In 2011, the US Congress passed the Food Safety Modernization Act, which tasks the US Food and Drug Administration to establish microbiological standards for agricultural water. However, little data are available for the microbiological quality of surface water irrigation supplies. During the 2015 irrigation season, we conducted a baseline study on the microbial water quality of large irrigation districts in California ( = 2) and Washington ( 4). Monthly samples ( 517) were analyzed for bacterial indicators (fecal coliforms, enterococci, and ) and pathogens ( spp., O157, and non-O157 Shiga toxin-producing [STEC]). Although there was a high degree of variability (μ ± SD = 59.13 ± 106.0), only 11% of samples (56/517) exceeded 126 colony-forming units (CFU) 100 mL, and only six samples exceeded 410 CFU 100 mL. Two volumes of water were collected for pathogen analysis (1 L and 10 L); prevalence of in 10-L samples (68149) was nearly double of that found in 1-L samples (132/517). We found STEC during ∼9% of sampling events (58/517); serotypes O26 and O45 were the most common at 31 and 26%, respectively. Pathogens were not associated with exceedance of the regulatory threshold, yet the odds of detecting increased approximately threefold (odds ration [O.R.] = 3.14, 0.0001) for every log increase in turbidity. Microbiological outcomes were highly district-specific, suggesting drivers of water quality vary across spatiotemporal scales. The true risk of contamination of produce from irrigation water supplies remains unknown, along with the optimal monitoring strategy to improve food safety.
切割产品中单核细胞增生李斯特氏菌的生长动力学。
影响因子：
2
作者：
Salazar,JoelleK;Sahu,SurasriN;Hildebrandt,IanM;Zhang,Lijie;Qi,Yan;Liggans,Girvin;Datta,AtinR;Tortorello,MaryLou
通讯作者：
Tortorello,MaryLou
接种在粪便浆料基质中时，罗马生菜上的大肠杆菌 O157:H7 失活。
影响因子：
2
作者：
Chase,JenniferA;Atwill,EdwardR;Partyka,MelissaL;Bond,RonaldF;Oryang,David
通讯作者：
Oryang,David