Rice bran mineral extract increases the expression of anagen-related molecules in human dermal papilla through wnt/catenin pathway.
Rice bran mineral extract increases the expression of anagen-related molecules in human dermal papilla through wnt/catenin pathway.
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DOI：
10.1080/16546628.2017.1412792
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发表时间：
2017
影响因子：
3.3
通讯作者：
Seo YK
中科院分区：
文献类型：
--
作者：
Kim YM;Kwon SJ;Jang HJ;Seo YK
本研究的目的是评估米糠矿物质提取物（RBM）增加人毛乳头（DOC）再生相关分子的表达。实验设阴性对照组、阳性对照组（抗坏血酸）、RBM组和OSA组。在DPC被给予各种处理后三天，蛋白质印迹分析显示，I型胶原表达在OSA组中增加2.5倍，在RBM组中增加4倍，并且ALP表达在OSA和RBM组中增加1.5倍，而纤连蛋白的表达在OSA组中增加约3倍，在RBM组中增加2.5倍。与对照组相比，OSA组和RBM组Wnt-3α和β-catenin蛋白表达均增加。此外，与阴性对照相比，OSA和RBM组中IL-1a的表达降低超过50%。通过RT-qPCR对mRNA表达的分析显示，I型胶原在OSA和RBM处理的DPC中增加1.2倍，而IV型胶原在OSA组中增加2.7倍，在RBM组中增加3.5倍。而TGF-β2 mRNA在OSA和RBM组分别下降约80%。DPC的多功能蛋白聚糖蛋白的免疫组织化学染色显示，与阴性对照相比，OSA和RBM处理组的DPC显著增加。因此，RBM具有恢复DPC活性和降低炎症相关标志物的潜力。可以预期，当RBM作为化妆品应用时，可以预期脱发预防和毛发生长增强。
The objective of this study is to evaluate of rice bran mineral extract (RBM) increases the expression of anagen-related molecules in human dermal papilla (DOCs). Four treatment groups were established to evaluate the efficacy of RBM, including a negative control, positive control (ascorbic acid), RBM and ortho-silicic acid (Si(OH)4) (OSA) group. Three days after the DPCs were administered the various treatments, western blot analysis showed that type I collagen expression was increased 2.5-fold in the OSA group and 4-fold in the RBM group, and ALP expression was increased 1.5-fold in the OSA and RBM group while the expression of fibronectin was increased ~3-fold in the OSA group and 2.5-fold in the RBM group. Also, the expression of Wnt-3α and β-catenin protein was increased in OSA and RBM group compared to control group. Furthermore, the expression of IL-1a was decreased by more than 50% in the OSA and RBM groups compared to the negative control. Analysis of mRNA expression by RT-qPCR showed that type I collagen increased 1.2-fold in the OSA- and RBM-treated DPCs, whereas type IV collagen increased 2.7-fold in the OSA group and 3.5-fold in the RBM group. However, TGF-β2 mRNA decreased about 80% in the OSA and RBM groups, respectively. Immunohistochemical staining of the DPCs for versican protein showed a significant increase in the OSA- and RBM-treated groups compared to the negative control. Thus, RBM have a potential to recover of DPCs activity and decreased inflammatory-related markers. It can be expected that hair loss prevention and hair growth enhancement can be expected when RBM is applied as a cosmetic product.
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影响因子：
3.7
作者：
Kim YE;Choi HC;Lee IC;Yuk DY;Lee H;Choi BY
通讯作者：
Choi BY
影响因子：
2.5
作者：
Iida, Machiko;Ihara, Setsunosuke;Matsuzaki, Takashi
通讯作者：
Matsuzaki, Takashi
影响因子：
3
作者：
Lobner, D
通讯作者：
Lobner, D
影响因子：
3.6
作者：
Boivin, Wendy A.;Jiang, Huijun;Hunt, David W. C.
通讯作者：
Hunt, David W. C.
影响因子：
2.7
作者：
COUCHMAN, JR;GIBSON, WT
通讯作者：
GIBSON, WT