An Elastic Mineralized 3D Electrospun PCL Nanofibrous Scaffold for Drug Release and Bone Tissue Engineering.
An Elastic Mineralized 3D Electrospun PCL Nanofibrous Scaffold for Drug Release and Bone Tissue Engineering.
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DOI：
10.1021/acsabm.1c00134
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发表时间：
2021-04-19
影响因子：
4.7
通讯作者：
Sun H
中科院分区：
文献类型：
--
作者：
Miszuk J;Liang Z;Hu J;Sanyour H;Hong Z;Fong H;Sun H
复杂形状和临界尺寸的骨缺损多年来一直是临床挑战。基于支架的策略如水凝胶提供局部药物释放，同时填充复杂的缺陷形状，但最终具有低机械强度的弱点，同时缺乏大孔和胶原蛋白模拟纳米纤维结构。因此，需要机械强度强的细胞外基质（ECM）模拟支架，其可以稳健地适应复杂形状的临界尺寸的缺陷，并同时提供局部的、持续的、多种生长因子释放。因此，我们开发了一种复合材料，双相PCL/羟基磷灰石（HA）3D纳米纤维（NF）支架骨组织再生通过使用我们的创新电纺为基础的热诱导自凝聚（TISA）技术。我们的ECM模拟TISA支架的一个有趣的特点是，即使在均匀涂覆矿物质后，它们也具有高度弹性和多孔性，并且可以很容易地压制以适应不同的缺损形状。此外，仿生矿物沉积技术允许我们同时封装不同类型的药物，例如，蛋白质和小分子，TISA支架在生理温和的条件下。与具有物理表面吸附的非那米（BMP 2信号激动剂）的支架相比，掺入的非那米复合支架显示较少的非那米突释和较长的持续释放，随后改善了体外细胞的成骨分化。总体而言，我们的研究表明，创新的压配式3D NF复合支架可能是多药物递送和骨组织工程的强大工具。
Complex shaped and critical-sized bone defects have been a clinical challenge for many years. Scaffold-based strategies such as hydrogels provide localized drug release while filling complex defect shapes, but ultimately possess weaknesses in low mechanical strength alongside a lack of macroporous and collagen-mimicking nanofibrous structures. Thus, there is a demand for mechanically strong, extracellular matrix (ECM) mimicking scaffolds that can robustly fit complex shaped critical sized defects and simultaneously provide localized, sustained, multiple growth factor release. We therefore developed a composite, bi-phasic PCL/hydroxyapatite (HA) 3D nanofibrous (NF) scaffold for bone tissue regeneration by using our innovative electrospun-based thermally induced self-agglomeration (TISA) technique. One intriguing feature of our ECM-mimicking TISA scaffolds is that they are highly elastic and porous even after evenly coated with minerals and can easily be pressed to fit different defect shapes. Furthermore, the bio-mimetic mineral deposition technique allowed us to simultaneously encapsulate different type of drugs, e.g., proteins and small molecules, on TISA scaffolds under physiologically mild conditions. Compared to scaffolds with physically surface-adsorbed phenamil, a BMP2 signaling agonist, incorporated phenamil composite scaffolds indicated less burst release and longer lasting sustained release of phenamil with subsequently improved osteogenic differentiation of cells in vitro. Overall, our study indicated that the innovative press-fit 3D NF composite scaffold may be a robust tool for multiple-drug delivery and bone tissue engineering.
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DOI：
10.12659/msm.892939
发表时间：
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期刊：
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发表时间：
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影响因子：
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