A comparison of blood factor XII autoactivation in buffer, protein cocktail, serum, and plasma solutions.
A comparison of blood factor XII autoactivation in buffer, protein cocktail, serum, and plasma solutions.
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DOI：
10.1016/j.biomaterials.2012.09.034
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发表时间：
2013-01
期刊：
影响因子：
14
通讯作者：
Vogler, Erwin A.
中科院分区：
文献类型：
--
作者：
Golas, Avantika;Yeh, Chyi-Huey Josh;Pitakjakpipop, Harit;Siedlecki, Christopher A.;Vogler, Erwin A.
通过与材料表面接触在体外激活血浆凝固明显依赖于血浆体积与激活剂表面积比。与目前对凝血级联生化的理解一致的唯一合理解释是，内源性途径激活复合物产生的促凝血刺激依赖于激活剂表面积。然而，本文显示溶解在缓冲液、蛋白质混合物、热变性血清和FXI缺陷血浆中的血液酶原因子XII（Hageman因子，FXII）的活化不表现出活化剂表面积依赖性。相反，测量到的是促凝剂-酶产率的高度可变的爆发，其表现出不可测量的动力学、对混合的敏感性或溶液-温度依赖性。因此，在缓冲液和含蛋白质的溶液中的FXII活化不表现出生化反应的特征，而是似乎是由FXII分子与亲水性活化剂颗粒相互作用诱导的“机械化学”反应，所述亲水性活化剂颗粒不从溶液中正式吸附血液蛋白。本研究的结果强烈表明，在血浆凝固的接触激活中观察到的激活剂表面积依赖性并不仅仅出现在内源性途径的FXII激活步骤。
Activation of blood plasma coagulation in vitro by contact with material surfaces is demonstrably dependent on plasma-volume-to-activator-surface-area ratio. The only plausible explanation consistent with current understanding of coagulation-cascade biochemistry is that procoagulant stimulus arising from the activation complex of the intrinsic pathway is dependent on activator surface area. And yet, it is herein shown that activation of the blood zymogen factor XII (Hageman factor, FXII) dissolved in buffer, protein cocktail, heat-denatured serum, and FXI deficient plasma does not exhibit activator surface-area dependence. Instead, a highly-variable burst of procoagulant-enzyme yield is measured that exhibits no measurable kinetics, sensitivity to mixing, or solution-temperature dependence. Thus, FXII activation in both buffer and protein-containing solutions does not exhibit characteristics of a biochemical reaction but rather appears to be a “mechanochemical” reaction induced by FXII molecule interactions with hydrophilic activator particles that do not formally adsorb blood proteins from solution. Results of this study strongly suggest that activator surface-area dependence observed in contact activation of plasma coagulation does not solely arise at the FXII activation step of the intrinsic pathway.
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影响因子：
14
作者：
Vogler, Erwin A.
通讯作者：
Vogler, Erwin A.
影响因子：
1.1
作者：
Woodhams, B;Girardot, O;Gourmelin, Y
通讯作者：
Gourmelin, Y
影响因子：
14
作者：
Golas, Avantika;Yeh, Chyi-Huey Joshua;Siedlecki, Christopher A.;Vogler, Erwin A.
通讯作者：
Vogler, Erwin A.
影响因子：
7.5
作者：
Alesci, Sonja;Borggrefe, Martin;Dempfle, Carl-Erik
通讯作者：
Dempfle, Carl-Erik
影响因子：
4.3
作者：
MARTORANA F;MORO A
通讯作者：
MORO A