Exosomal miR-1290 is a potential biomarker of high-grade serous ovarian carcinoma and can discriminate patients from those with malignancies of other histological types.
Exosomal miR-1290 is a potential biomarker of high-grade serous ovarian carcinoma and can discriminate patients from those with malignancies of other histological types.
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DOI：
10.1186/s13048-018-0458-0
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发表时间：
2018-09-15
影响因子：
4
通讯作者：
Kimura T
中科院分区：
文献类型：
--
作者：
Kobayashi M;Sawada K;Nakamura K;Yoshimura A;Miyamoto M;Shimizu A;Ishida K;Nakatsuka E;Kodama M;Hashimoto K;Mabuchi S;Kimura T
MicroRNAs(MiRNAs)稳定地存在于循环血液中，包裹在胞外囊泡中，如外体；因此，血清miRNAs具有作为新的癌症生物标志物的潜力。迫切需要新的诊断标志物来检测高级别浆液性卵巢癌(HGSOC)。本研究的目的是鉴定HGSOC特异的miRNA，并分析血清miRNA能否将HGSOC患者与健康对照组或其他组织学类型的卵巢恶性肿瘤患者区分开来。从卵巢癌细胞系中收集外体，提取外体miRNAs。MiRNA微阵列分析显示，HGSOC特异的几个miRNAs水平升高。其中，我们重点研究了miR-1290。收集70例卵巢癌患者和13例健康人血清，用实时定量聚合酶链式反应检测其表达水平。与其他组织学类型的卵巢癌患者不同，HGSOC患者血清miR-1290的表达显著高于健康对照组(3.52倍；P = 0.03)。晚期肝癌miR-1290的相对表达高于早期(4.23vs.1.58；P = 0.23)。术后其表达水平显著下降(P<0.0 1)，表明 和 反映了肿瘤负荷。受试者工作特征曲线分析显示，以1.20作为临界值，鉴别HGSOC(AUC = 0.71)的敏感度和特异度分别为63%和85%；以1.55为临界值，鉴别HGSOC(AUC = 0.76)和其他组织学类型恶性肿瘤的敏感度和特异度分别为47%和85%。血清miR-1290在HGSOC患者中显著升高，可用于区分HGSOC患者和其他组织学类型的恶性肿瘤患者，是一种新的潜在的HGSOC诊断生物标志物。
microRNAs (miRNAs) stably exist in circulating blood encapsulated in extracellular vesicles such as exosomes; therefore, serum miRNAs have the potential to serve as novel cancer biomarkers. New diagnostic markers to detect high grade serous ovarian cancer (HGSOC) are urgently needed. The aim of this study was to identify miRNAs specific to HGSOC and analyze whether serum miRNA can discriminate HGSOC patients from healthy controls or patients with ovarian malignancies of other histological types. Exosomes from ovarian cancer cell lines were collected and exosomal miRNAs extracted. miRNA microarray analysis revealed several elevated miRNAs specific to HGSOC. Among these, we focused on miR-1290. Sera from 70 ovarian cancer patients and 13 healthy controls were gathered and its expression levels detected by quantitative real-time polymerase chain reaction. In HGSOC patients, serum miR-1290 was significantly overexpressed compared to in healthy controls (3.52 fold; P = 0.03), unlike in patients with ovarian cancers of other histological types. The relative expression of miR-1290 was higher in advanced stages of HGSOC than in early stages (4.23 vs. 1.58; P = 0.23). Its expression significantly decreased after operation (5.87 to 1.17; P < 0.01), indicating that this miRNA reflects tumor burden. A receiver operating characteristic curve analysis showed that at the cut-off of 1.20, the sensitivity and specificity were 63% and 85% respectively for discriminating patients with HGSOC (area under the curve [AUC] = 0.71) from healthy controls, and at the cut-off of 1.55, the sensitivity and specificity were 47% and 85% respectively for discriminating patients with HGSOC (AUC = 0.76) from those with malignancies of other histological types. Serum miR-1290 is significantly elevated in patients with HGSOC and can be used to discriminate these patients from those with malignancies of other histological types; it is a new potential diagnostic biomarker for HGSOC.
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影响因子：
16
作者：
Lötvall J;Hill AF;Hochberg F;Buzás EI;Di Vizio D;Gardiner C;Gho YS;Kurochkin IV;Mathivanan S;Quesenberry P;Sahoo S;Tahara H;Wauben MH;Witwer KW;Théry C
通讯作者：
Théry C
DOI：
10.1158/1055-9965.epi-15-1299
发表时间：
2016-09
期刊：
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
影响因子：
--
作者：
Leung F;Bernardini MQ;Brown MD;Zheng Y;Molina R;Bast RC Jr;Davis G;Serra S;Diamandis EP;Kulasingam V
通讯作者：
Kulasingam V
影响因子：
3.7
作者：
Ogata-Kawata H;Izumiya M;Kurioka D;Honma Y;Yamada Y;Furuta K;Gunji T;Ohta H;Okamoto H;Sonoda H;Watanabe M;Nakagama H;Yokota J;Kohno T;Tsuchiya N
通讯作者：
Tsuchiya N
影响因子：
9.7
作者：
Cohen, Marie;Petignat, Patrick
通讯作者：
Petignat, Patrick
影响因子：
4.7
作者：
Moore, Richard G.;Brown, Amy K.;Bast, Robert C., Jr.
通讯作者：
Bast, Robert C., Jr.