Opaque7 Encodes an Acyl-Activating Enzyme-Like Protein That Affects Storage Protein Synthesis in Maize Endosperm
Opaque7 Encodes an Acyl-Activating Enzyme-Like Protein That Affects Storage Protein Synthesis in Maize Endosperm
复制标题
Opaque7 编码一种影响玉米胚乳中储存蛋白合成的酰基激活酶样蛋白
DOI：
10.1534/genetics.111.133967
复制
发表时间：
2011-12
期刊：
影响因子：
3.3
通讯作者：
Song, Rentao
中科院分区：
文献类型：
--
作者：
Lai, Jinsheng;Zhu, Lihuang;Xu, Zhengkai;Song, Rentao
在玉米中，一系列淀粉胚乳突变体可以通过降低胚乳中主要贮藏蛋白玉米醇溶蛋白的含量来提高赖氨酸含量。这些突变体的克隆和鉴定可以揭示玉米胚乳中玉米醇溶蛋白积累的调控机制。Opaque 7（o 7）是一个典型的玉米淀粉胚乳突变体，对玉米醇溶蛋白积累和高赖氨酸含量有很大影响。本研究通过图位克隆的方法克隆了O 7基因，并通过转基因功能互补和RNA干扰进行了证实。o 7-ref等位基因具有12-bp的框内缺失。4个氨基酸的缺失导致o 7蛋白在体内的低积累。O 7基因编码与AAE 3高度相似的酰基活化酶。o 7突变体的不透明表型是由α-玉米醇溶蛋白浓度降低引起的蛋白体大小和数量减少而产生的。氨基酸和代谢产物分析表明，O 7基因可能通过影响α-酮基谷氨酸和草酰乙酸来影响氨基酸的合成。含有靶向玉米O 7的水稻直系同源物的RNAi构建体的转基因水稻种子也产生较少量的种子蛋白，并显示不透明的胚乳表型，表明O 7在谷类作物中的保守生物学功能。O 7基因的克隆揭示了一种新的贮藏蛋白合成调控机制，为禾谷类种子贮藏蛋白含量的遗传调控提供了一个有效的靶点。
In maize, a series of seed mutants with starchy endosperm could increase the lysine content by decreased amount of zeins, the main storage proteins in endosperm. Cloning and characterization of these mutants could reveal regulatory mechanisms for zeins accumulation in maize endosperm. Opaque7 (o7) is a classic maize starchy endosperm mutant with large effects on zeins accumulation and high lysine content. In this study, the O7 gene was cloned by map-based cloning and confirmed by transgenic functional complementation and RNAi. The o7-ref allele has a 12-bp in-frame deletion. The four-amino-acid deletion caused low accumulation of o7 protein in vivo. The O7 gene encodes an acyl-activating enzyme with high similarity to AAE3. The opaque phenotype of the o7 mutant was produced by the reduction of protein body size and number caused by a decrease in the α-zeins concentrations. Analysis of amino acids and metabolites suggested that the O7 gene might affect amino acid biosynthesis by affecting α-ketoglutaric acid and oxaloacetic acid. Transgenic rice seeds containing RNAi constructs targeting the rice ortholog of maize O7 also produced lower amounts of seed proteins and displayed an opaque endosperm phenotype, indicating a conserved biological function of O7 in cereal crops. The cloning of O7 revealed a novel regulatory mechanism for storage protein synthesis and highlighted an effective target for the genetic manipulation of storage protein contents in cereal seeds.
登录
查看更多内容
影响因子：
4.4
作者：
Hartings H;Lauria M;Lazzaroni N;Pirona R;Motto M
通讯作者：
Motto M
影响因子：
56.9
作者：
P. Misra;R. Jambunathan;E. Mertz;D. V. Glover;H. M. Barbosa;Kenneth S. McWhirter
通讯作者：
P. Misra;R. Jambunathan;E. Mertz;D. V. Glover;H. M. Barbosa;Kenneth S. McWhirter
DOI：
10.1111/j.1432-1033.1996.0468h.x
发表时间：
1996-09-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Blobel, F;Erdmann, R
通讯作者：
Erdmann, R
影响因子：
4.8
作者：
Black, PN;Zhang, Q;DiRusso, CC
通讯作者：
DiRusso, CC
从淀粉含量高的谷物种子中分离出高质量 RNA
影响因子：
3.3
作者：
Wang, Guifeng;Wang, Gang;Song, Rentao
通讯作者：
Song, Rentao