The Zea mays mutants opaque-2 and opaque-7 disclose extensive changes in endosperm metabolism as revealed by protein, amino acid, and transcriptome-wide analyses.
The Zea mays mutants opaque-2 and opaque-7 disclose extensive changes in endosperm metabolism as revealed by protein, amino acid, and transcriptome-wide analyses.
复制标题
DOI：
10.1186/1471-2164-12-41
复制
发表时间：
2011-01-18
期刊：
影响因子：
4.4
通讯作者：
Motto M
中科院分区：
文献类型：
--
作者：
Hartings H;Lauria M;Lazzaroni N;Pirona R;Motto M
玉米（Zea mays L.）谷物成熟已得到很好的确立。然而，关键的分子决定因素控制碳通量的粮食和分配的碳淀粉和蛋白质更难以捉摸。Opaque-2（O2）基因是玉米胚乳发育过程中碳水化合物、氨基酸和贮藏蛋白代谢整合的一个很好的例子，是植物中最具代表性的转录因子之一。证据还表明，Opaque-7（O 7）基因在影响胚乳代谢中起作用。本研究的重点是通过评估蛋白质和氨基酸组成以及转录组分析来评估o2和o 7突变对玉米胚乳代谢诱导的变化，以研究这两个基因在单突变体和双突变体中的功能相互作用。我们表明，分析的突变体的整体氨基酸组成相似。每个突变体相对于野生型具有高的赖氨酸和降低的Glx和Leu含量。基因表达谱，基于一个unigene集组成的7,250 EST，使我们能够确定一系列的music相关的下调（17.1%）和上调（3.2%）的成绩单。几个差异表达的EST同源的基因编码的酶参与氨基酸合成，碳代谢（TCA循环和糖酵解），在存储蛋白质和淀粉代谢，在基因转录和翻译过程中，在信号转导，蛋白质，脂肪酸和脂质合成。我们的分析表明，所研究的突变体是多效性的，并在几个胚乳相关的代谢过程中发挥关键作用。多效性效应在o 7突变体中不太明显，但在o2和o2o 7背景中很严重，基因表达模式发生了很大变化，影响了广泛的核表达基因。虽然，必要的，本文是描述性的，需要更多的工作来定义基因的功能和解剖基因表达的复杂调控，基因分离和表征的日期给我们一个有趣的深入了解胚乳代谢的机制。
The changes in storage reserve accumulation during maize (Zea mays L.) grain maturation are well established. However, the key molecular determinants controlling carbon flux to the grain and the partitioning of carbon to starch and protein are more elusive. The Opaque-2 (O2) gene, one of the best-characterized plant transcription factors, is a good example of the integration of carbohydrate, amino acid and storage protein metabolisms in maize endosperm development. Evidence also indicates that the Opaque-7 (O7) gene plays a role in affecting endosperm metabolism. The focus of this study was to assess the changes induced by the o2 and o7 mutations on maize endosperm metabolism by evaluating protein and amino acid composition and by transcriptome profiling, in order to investigate the functional interplay between these two genes in single and double mutants. We show that the overall amino acid composition of the mutants analyzed appeared similar. Each mutant had a high Lys and reduced Glx and Leu content with respect to wild type. Gene expression profiling, based on a unigene set composed of 7,250 ESTs, allowed us to identify a series of mutant-related down (17.1%) and up-regulated (3.2%) transcripts. Several differentially expressed ESTs homologous to genes encoding enzymes involved in amino acid synthesis, carbon metabolism (TCA cycle and glycolysis), in storage protein and starch metabolism, in gene transcription and translation processes, in signal transduction, and in protein, fatty acid, and lipid synthesis were identified. Our analyses demonstrate that the mutants investigated are pleiotropic and play a critical role in several endosperm-related metabolic processes. Pleiotropic effects were less evident in the o7 mutant, but severe in the o2 and o2o7 backgrounds, with large changes in gene expression patterns, affecting a broad range of kernel-expressed genes. Although, by necessity, this paper is descriptive and more work is required to define gene functions and dissect the complex regulation of gene expression, the genes isolated and characterized to date give us an intriguing insight into the mechanisms underlying endosperm metabolism.
登录
查看更多内容
影响因子：
5.1
作者：
Bhat, RA;Borst, JW;Thompson, RD
通讯作者：
Thompson, RD
影响因子：
7.4
作者：
Forestan, Cristian;Meda, Silvia;Varotto, Serena
通讯作者：
Varotto, Serena
影响因子：
7.4
作者：
BROCHETTOBRAGA, MR;LEITE, A;ARRUDA, P
通讯作者：
ARRUDA, P
DOI：
10.1007/s004380050657
发表时间：
1998-02-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
Damerval, C;Le Guilloux, M
通讯作者：
Le Guilloux, M
影响因子：
7.4
作者：
Dai, Mingqiu;Hu, Yongfeng;Zhou, Dao-Xiu
通讯作者：
Zhou, Dao-Xiu