A role for Dictyostelium racE in cortical tension and cleavage furrow progression.
A role for Dictyostelium racE in cortical tension and cleavage furrow progression.
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DOI：
10.1083/jcb.141.2.483
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发表时间：
1998-04-20
期刊：
影响因子：
--
通讯作者：
De Lozanne A
中科院分区：
文献类型：
--
作者：
Gerald N;Dai J;Ting-Beall HP;De Lozanne A
小GT受体racE在网骨藻的胞质分裂中是必需的。我们发现，这一要求仅限于悬浮培养的细胞。当附着在基质上时，racE无效细胞形成肌动球蛋白收缩环，并正常完成胞质分裂。尽管如此，当在悬浮液中时，racE无效细胞在胞质分裂中完全失败。为了理解这种条件要求racE，我们开发了一种方法来观察悬浮液中的胞质分裂。使用这种方法，我们发现，racE空细胞试图在悬浮液中通过形成收缩环和卵裂沟胞质分裂。然而，细胞形成多个泡，并且由于卵裂沟的退化而在胞质分裂中失败。我们认为这种表型是由与野生型细胞相比，在racE无效细胞中发现的极低水平的皮质张力引起的。racE无效细胞的皮质张力降低不是由它们的F-肌动蛋白含量减少引起的。相反，有丝分裂racE空细胞含有异常的F-肌动蛋白聚集体。这些结果表明，racE是必不可少的组织的皮质细胞骨架，以保持适当的皮质完整性。racE的这一功能不依赖于与底物的附着，但可以被由与底物的粘附诱导的其他信号传导途径绕过。
The small GTPase racE is essential for cytokinesis in Dictyostelium. We found that this requirement is restricted to cells grown in suspension. When attached to a substrate, racE null cells form an actomyosin contractile ring and complete cytokinesis normally. Nonetheless, racE null cells fail completely in cytokinesis when in suspension. To understand this conditional requirement for racE, we developed a method to observe cytokinesis in suspension. Using this approach, we found that racE null cells attempt cytokinesis in suspension by forming a contractile ring and cleavage furrow. However, the cells form multiple blebs and fail in cytokinesis by regression of the cleavage furrow. We believe this phenotype is caused by the extremely low level of cortical tension found in racE null cells compared to wild-type cells. The reduced cortical tension of racE null cells is not caused by a decrease in their content of F-actin. Instead, mitotic racE null cells contain abnormal F-actin aggregates. These results suggest that racE is essential for the organization of the cortical cytoskeleton to maintain proper cortical integrity. This function of racE is independent of attachment to a substrate, but can be bypassed by other signaling pathways induced by adhesion to a substrate.
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影响因子：
7.8
作者：
Fukui, Y;De Lozanne, A;Spudich, J A
通讯作者：
Spudich, J A
DOI：
10.1083/jcb.120.5.1187
发表时间：
1993-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kishi K;Sasaki T;Kuroda S;Itoh T;Takai Y
通讯作者：
Takai Y
影响因子：
7.8
作者：
Adachi, H;Takahashi, Y;Sutoh, K
通讯作者：
Sutoh, K
使用二氢细胞藻蛋白B延迟HeLa细胞裂解到相间的延迟：有丝分裂后纺锤体和末期盘的保留与同步裂解恢复相关。
影响因子：
7.8
作者：
MARTINEAU, SN;ANDREASSEN, PR;MARGOLIS, RL
通讯作者：
MARGOLIS, RL
DOI：
10.1073/pnas.80.18.5622
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
KEESE, CR;GIAEVER, I
通讯作者：
GIAEVER, I