Delay of HeLa cell cleavage into interphase using dihydrocytochalasin B: retention of a postmitotic spindle and telophase disc correlates with synchronous cleavage recovery.
Delay of HeLa cell cleavage into interphase using dihydrocytochalasin B: retention of a postmitotic spindle and telophase disc correlates with synchronous cleavage recovery.
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使用二氢细胞藻蛋白B延迟HeLa细胞裂解到相间的延迟：有丝分裂后纺锤体和末期盘的保留与同步裂解恢复相关。
DOI：
10.1083/jcb.131.1.191
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发表时间：
1995-10
影响因子：
7.8
通讯作者：
MARGOLIS, RL
中科院分区：
文献类型：
--
作者：
MARTINEAU, SN;ANDREASSEN, PR;MARGOLIS, RL
在哺乳动物细胞胞质分裂过程中，决定分裂沟位置和时间的分子信号目前尚不清楚。我们详细研究了二氢细胞松弛素B（DCB），一种药物，干扰肌动蛋白组装，对特定的晚期有丝分裂同步HeLa细胞的事件的影响。当在10 μ M DCB下阻断卵裂沟形成时，细胞返回到间期，其标准为核与核纤层蛋白边界的重组、p34 cdc 2激酶的细胞周期蛋白B组分的降解和有丝分裂特异性MPM-2抗原的丧失。然而，当分裂不能进行时，细胞分裂的机制保留长达一小时进入G1。保留的组件主要包括一个“有丝分裂后”的纺锤体和一个末期盘，模板的有丝分裂纺锤体在后期，可以确定的位置和时间的分裂沟的结构。从DCB阻断释放后，G1细胞进行快速和同步的分裂。我们的结论是，有丝分裂纺锤体不可避免地被破坏，在有丝分裂结束时，但持续作为一个整体结构与分裂末期光盘的情况下。我们还得出结论，细胞分裂可以发生在G1期，因此是一个事件代谢独立的有丝分裂。保留的末期圆盘可能确实是沟形成的位置信号，因为G1裂解仅发生在保留的圆盘位于细胞皮层下方的位置。我们所描述的协议，现在应该能够开发一个模型系统的研究哺乳动物细胞分裂作为一个独立的有丝分裂的同步事件。
The molecular signals that determine the position and timing of the cleavage furrow during mammalian cell cytokinesis are presently unknown. We have studied in detail the effect of dihydrocytochalasin B (DCB), a drug that interferes with actin assembly, on specific late mitotic events in synchronous HeLa cells. When cleavage furrow formation is blocked at 10 microM DCB, cells return to interphase by the criteria of reformation of nuclei with lamin borders, degradation of the cyclin B component of p34cdc2 kinase, and loss of mitosis specific MPM-2 antigens. However, the machinery for cell cleavage is retained for up to one hour into G1 when cleavage cannot proceed. The components retained consist prominently of a "postmitotic" spindle and a telophase disc, a structure templated by the mitotic spindle in anaphase that may determine the position and timing of the cleavage furrow. Upon release from DCB block, G1 cells proceed through a rapid and synchronous cleavage. We conclude that the mitotic spindle is not inevitably destroyed at the end of mitosis, but persists as an integral structure with the telophase disc in the absence of cleavage. We also conclude that cell cleavage can occur in G1, and is therefore an event metabolically independent of mitosis. The retained telophase disc may indeed signal the position of furrow formation, as G1 cleavage occurs only in the position where the retained disc underlies the cell cortex. The protocol we describe should now enable development of a model system for the study of mammalian cell cleavage as a synchronous event independent of mitosis.
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DOI：
10.1083/jcb.115.1.1
发表时间：
1991-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Pines J;Hunter T
通讯作者：
Hunter T
DOI：
10.1083/jcb.116.3.707
发表时间：
1992-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hunt T;Luca FC;Ruderman JV
通讯作者：
Ruderman JV
影响因子：
11.4
作者：
BAILLY, E;DOREE, M;BORNENS, M
通讯作者：
BORNENS, M
DOI：
10.1083/jcb.127.3.789
发表时间：
1994-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Andreassen PR;Margolis RL
通讯作者：
Margolis RL
影响因子：
64.8
作者：
NISLOW, C;LOMBILLO, VA;MCINTOSH, JR
通讯作者：
MCINTOSH, JR