Genome-Wide Identification and Comparative Profiling of MicroRNAs Reveal Flavonoid Biosynthesis in Two Contrasting Flower Color Cultivars of Tree Peony.
Genome-Wide Identification and Comparative Profiling of MicroRNAs Reveal Flavonoid Biosynthesis in Two Contrasting Flower Color Cultivars of Tree Peony.
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全基因组鉴定和 microRNA 比较分析揭示了两个对比花色牡丹品种中的类黄酮生物合成
DOI：
10.3389/fpls.2021.797799
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发表时间：
2021
影响因子：
5.6
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Luo X;Luo S;Fu Y;Kong C;Wang K;Sun D;Li M;Yan Z;Shi Q;Zhang Y
microRNA（miRNA）介导的基因调控参与植物的多种生理过程。花色是牡丹的重要观赏性状之一。然而，黄花牡丹品种特别罕见。为了阐明牡丹黄色色素形成的miRNA调控机制，本研究采用色素鉴定、miRNA鉴定、表达分析和基因功能验证相结合的方法，对两个花色品种“High Noon”和“Roufurong”进行了研究。黄酮/黄酮醇类化合物和花色苷类化合物分别是“正午”和“肉芙蓉”花瓣着色的主要贡献者。随后，基于现有基因组数据的miRNA分析鉴定了9个差异表达的miRNA和12个涉及类黄酮生物合成的相关靶基因。它们的动态表达模式决定了mdm-miR 156 b-PsSPL 2模块在牡丹花黄色着色中的关键作用。序列分析和亚细胞定位证实PsSPL 2可能是一个核定位的转录因子。PsSPL 2基因在烟草中的过表达导致花色素苷含量的降低和NtF 3 ′H和NtDFR转录本的下调。PsSPL 2基因沉默的花瓣颜色变淡，THC、Ap和Ch含量显著降低。同时，PsSPL 2基因沉默的花瓣中PsCHS、PsCHI和PsF 3 H的表达水平显著降低，而PsF 3 ′H和PsDFR的表达水平显著升高。本研究为深入了解牡丹黄化相关的miRNA调控网络提供了新的视角，并为牡丹黄化过程中的生理变化提供了有价值的信息。
MicroRNA (miRNA)-mediated gene regulation is involved in various physiological processes in plants. Flower color is one of the vital ornamental traits of tree peony (Paeonia suffruticosa Andr.). However, the yellow-flowered tree peony cultivars are particularly rare. To elucidate the miRNA-mediated gene regulatory mechanism underlying yellow pigmentation in tree peony, we combined pigment assessment, miRNA identification, expression analysis, and gene functional verification in two contrasting flower color cultivars “High Noon” and “Roufurong.” Flavones/flavonols and anthocyanins were found to be the main contributors to the coloration of “High Noon” and “Roufurong” petals, respectively. Subsequently, miRNA analysis based on available genome data identified 9 differentially expressed miRNAs and 12 relevant target genes implicated in flavonoid biosynthesis. Their dynamic expression patterns determined the key role of mdm-miR156b-PsSPL2 module in yellow pigmentation of tree peony flowers. The sequence analysis and subcellular localization validated that PsSPL2 might function as a nuclear-localized transcription factor. Overexpression of PsSPL2 in tobacco resulted in a decrease of anthocyanin content and down-regulation of NtF3′H and NtDFR transcripts. PsSPL2-silenced petals exhibited lighter yellow color, and the contents of THC, Ap, and Ch decreased significantly. Meanwhile, expression levels of PsCHS, PsCHI, and PsF3H were significantly decreased in the petals with PsSPL2 silencing, while those of PsF3′H and PsDFR were remarkably increased. This study offers a novel insight into yellow pigmentation-related miRNA regulation network in tree peony, and further provides the valuable information on physiological changes during yellow coloring process of tree peony.
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影响因子：
5.6
作者：
Fan, Jinling;Zhu, Wenxue;Tao, Guanjun
通讯作者：
Tao, Guanjun
影响因子：
--
作者：
Langmead, Ben
通讯作者：
Langmead, Ben
影响因子：
5.6
作者：
Gao L;Yang H;Liu H;Yang J;Hu Y
通讯作者：
Hu Y
影响因子：
5.6
作者：
Cao S;Zhu QH;Shen W;Jiao X;Zhao X;Wang MB;Liu L;Singh SP;Liu Q
通讯作者：
Liu Q
影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
通讯作者：
Carrington JC