Comparative profiling of miRNA expression in developing seeds of high linoleic and high oleic safflower (Carthamus tinctorius L.) plants.
Comparative profiling of miRNA expression in developing seeds of high linoleic and high oleic safflower (Carthamus tinctorius L.) plants.
复制标题
DOI：
10.3389/fpls.2013.00489
复制
发表时间：
2013
影响因子：
5.6
通讯作者：
Liu Q
中科院分区：
文献类型：
--
作者：
Cao S;Zhu QH;Shen W;Jiao X;Zhao X;Wang MB;Liu L;Singh SP;Liu Q
富含油酸的植物油因其更好的营养价值和潜在的工业应用而被认为是有优势的。经典红花油中的油酸含量通常为10-15%，而天然突变株(Ol)在种子油中积累的油酸含量高达70%。作为红花高油酸(HO)性状分子特征研究的一部分，我们利用深度测序技术分析了红花种子发育过程中表达ol等位基因的microRNA(MiRNA)群体，并与野生型高油酸(HL)红花进行了比较。纯合olHo和野生型HL红花中成熟胚胎的小RNA群体大小分布模式非常相似，但仅有约16.5%的独特小RNA在这两种类型中重叠。从这两个小RNA群体中，我们发现了55个已知的miRNAs，并确定了两个候选的新的miRNA家族，它们可能是红花种子发育中所特有的。对于保守的miRNAs，预测了具有保守和新功能的靶基因。我们还鉴定了HO和HL型红花之间差异表达的13个miRNAs。这些结果可能为解开miRNA介导的调控红花油酸积累和红花胚胎发育过程的分子过程奠定基础。
Vegetable oils high in oleic acid are considered to be advantageous because of their better nutritional value and potential industrial applications. The oleic acid content in the classic safflower oil is normally 10–15% while a natural mutant (ol) accumulates elevated oleic acid up to 70% in seed oil. As a part of our investigation into the molecular features of the high oleic (HO) trait in safflower we have profiled the microRNA (miRNA) populations in developing safflower seeds expressing the ol allele in comparison to the wild type high linoleic (HL) safflower using deep sequencing technology. The small RNA populations of the mid-maturity developing embryos of homozygous ol HO and wild type HL safflower had a very similar size distribution pattern, however, only ~16.5% of the unique small RNAs were overlapping in these two genotypes. From these two small RNA populations we have found 55 known miRNAs and identified two candidate novel miRNA families to be likely unique to the developing safflower seeds. Target genes with conserved as well as novel functions were predicted for the conserved miRNAs. We have also identified 13 miRNAs differentially expressed between the HO and HL safflower genotypes. The results may lay a foundation for unraveling the miRNA-mediated molecular processes that regulate oleic acid accumulation in the HO safflower mutant and developmental processes in safflower embryos in general.
登录
查看更多内容
影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
4.3
作者：
Li, Haiyan;Dong, Yuanyuan;Li, Xiaokun
通讯作者：
Li, Xiaokun
影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
通讯作者：
Carrington JC
影响因子：
16
作者：
Jones-Rhoades, MW;Bartel, DP
通讯作者：
Bartel, DP
影响因子：
4.5
作者：
Dolgosheina, Elena V.;Morin, Ryan D.;Unrau, Peter J.
通讯作者：
Unrau, Peter J.