Molecular Detection of Mycobacterium tuberculosis in Oral Mucosa from Patients with Presumptive Tuberculosis.
Molecular Detection of Mycobacterium tuberculosis in Oral Mucosa from Patients with Presumptive Tuberculosis.
复制标题
DOI：
10.3390/jcm9124124
复制
发表时间：
2020-12-21
影响因子：
3.9
通讯作者：
Dominguez J
中科院分区：
文献类型：
--
作者：
Molina-Moya B;Ciobanu N;Hernandez M;Prat-Aymerich C;Crudu V;Adams ER;Codreanu A;Sloan DJ;Cuevas LE;Dominguez J
结核病（TB）的诊断越来越多地基于痰中结核分枝杆菌复合群（MTBC）DNA的检测，使用分子诊断测试作为诊断的第一测试。然而，在儿童、无排痰性咳嗽的患者和老年人中，痰液可能难以获得，因此需要测试非痰液样本的方法。我们评估了是否可以从结核病患者的口腔粘膜中检测到结核病。使用培养、Xpert MTB/RIF、涂片显微镜和X射线检查疑似TB的成人。在PrimeStore-MTM上采集口腔粘膜拭子，如果在30天内进行检测，则在室温下储存，如果在以后进行检测，则在−20 °C下储存。RT-PCR检测M.结核病DNA 80名患者有细菌学确诊的结核病，34名患者有细菌学阴性的结核病（阴性测试，但异常的X射线），152名患者被认为没有结核病（非结核病）。口腔拭子RT-PCR在细菌学确诊的29/80（36.3%）、细菌学阴性的9/34（26.5%）和非TB的29/152（19.1%）中呈阳性。储存少于和超过30天的样本之间的产量变化（p = 0.013），细菌学确认的样本为61%（11/18）和29%（18/62），细菌学阴性的参与者为30.8%（4/13）和23.8%（5/21）。在非TB患者中，特异性为80.9%（123/152），在储存少于30天的样品中为78.3%（18/23），在储存超过30天的样品中为81.4%（105/129）（p = 0.46）。对M.口腔粘膜标本中结核杆菌的检测是可行的，但储存条件可能会影响产量。
Tuberculosis (TB) diagnosis is increasingly based on the detection of Mycobacterium tuberculosis complex (MTBC) DNA in sputum using molecular diagnostic tests as the first test for diagnosis. However, sputum can be difficult to obtain in children, patients without productive cough, and the elderly and approaches testing non-sputum samples are needed. We evaluated whether TB can be detected from the oral mucosa of patients with TB. Adults with presumptive TB were examined using culture, Xpert MTB/RIF, smear microscopy and X-Rays. Oral mucosa swabs collected on PrimeStore-MTM, stored at room temperature if tested within 30 days or at −20 °C if examined at a later time. RT-PCR was performed to detect M. tuberculosis DNA. Eighty patients had bacteriologically-confirmed TB, 34 had bacteriologically-negative TB (negative tests but abnormal X-rays) and 152 were considered not to have TB (not TB). Oral swabs RT-PCR were positive in 29/80 (36.3%) bacteriologically-confirmed, 9/34 (26.5%) bacteriologically-negative and 29/152 (19.1%) not TB. The yield varied among samples stored for less and more than 30 days (p = 0.013) from 61% (11/18) and 29% (18/62) among bacteriologically confirmed, and 30.8% (4/13) and 23.8% (5/21) among bacteriologically-negative participants. Among not TB patients, the specificity was 80.9% (123/152), being 78.3% (18/23) among samples stored less than 30 days and 81.4% (105/129) among samples stored for more than 30 days (p = 0.46). The detection of M. tuberculosis in oral mucosa samples is feasible, but storage conditions may affect the yield.
登录
查看更多内容
影响因子：
9.4
作者：
Luabeya, Angelique K.;Wood, Rachel C.;Cangelosi, Gerard A.
通讯作者：
Cangelosi, Gerard A.
影响因子：
1.4
作者：
Dwyer-Hemmings, Louis
通讯作者：
Dwyer-Hemmings, Louis
影响因子：
3.3
作者：
Omar, Shaheed V.;Peters, Remco P. H.;Fourie, P. Bernard
通讯作者：
Fourie, P. Bernard
DOI：
10.5588/ijtld.14.0769
发表时间：
2015-05-01
影响因子：
4
作者：
Daum, L. T.;Peters, R. P. H.;Fischer, G. W.
通讯作者：
Fischer, G. W.
影响因子：
4.6
作者：
Wood RC;Luabeya AK;Weigel KM;Wilbur AK;Jones-Engel L;Hatherill M;Cangelosi GA
通讯作者：
Cangelosi GA