Detection of Mycobacterium tuberculosis DNA on the oral mucosa of tuberculosis patients.
Detection of Mycobacterium tuberculosis DNA on the oral mucosa of tuberculosis patients.
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DOI：
10.1038/srep08668
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发表时间：
2015-03-02
影响因子：
4.6
通讯作者：
Cangelosi GA
中科院分区：
文献类型：
--
作者：
Wood RC;Luabeya AK;Weigel KM;Wilbur AK;Jones-Engel L;Hatherill M;Cangelosi GA
肺结核（TB）的诊断通常包括痰液的实验室分析，痰液是一种来自活动性疾病患者气道深处的粘性物质。作为诊断样本基质，痰液可能难以通过微生物学和分子技术收集和分析。另一种侵入性较小的样本矩阵可以大大简化结核病诊断。我们假设结核分枝杆菌细胞或DNA积聚在肺结核患者的口腔上皮上，并且可以通过使用口腔（颊）拭子收集和检测。为了检验这一假设，从20名活动性肺结核受试者和20名健康对照者中各采集3份拭子。采用特异性聚合酶链反应（PCR）检测样本。结核病IS6110插入元件。20例确诊病例受试者中的18例（90%）至少获得2份阳性拭子。健康对照样品为100%阴性。本病例对照研究支持了过去关于M。口腔拭子中的结核病DNA检测。口腔拭子样本是非侵入性的，非粘性的，容易收集，无论是否有活动性结核病症状。这些特征可能使更简单和更积极的结核病病例发现策略成为可能。
Diagnosis of pulmonary tuberculosis (TB) usually includes laboratory analysis of sputum, a viscous material derived from deep in the airways of patients with active disease. As a diagnostic sample matrix, sputum can be difficult to collect and analyze by microbiological and molecular techniques. An alternative, less invasive sample matrix could greatly simplify TB diagnosis. We hypothesized that Mycobacterium tuberculosis cells or DNA accumulate on the oral epithelia of pulmonary TB patients, and can be collected and detected by using oral (buccal) swabs. To test this hypothesis, 3 swabs each were collected from 20 subjects with active pulmonary TB and from 20 healthy controls. Samples were tested by using a polymerase chain reaction (PCR) specific to the M. tuberculosis IS6110 insertion element. Eighteen out of 20 confirmed case subjects (90%) yielded at least 2 positive swabs. Healthy control samples were 100% negative. This case-control study supports past reports of M. tuberculosis DNA detection in oral swabs. Oral swab samples are non-invasive, non-viscous, and easy to collect with or without active TB symptoms. These characteristics may enable simpler and more active TB case finding strategies.
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DOI：
10.1016/s1473-3099(13)70008-2
发表时间：
2013-04
期刊：
The Lancet. Infectious diseases
影响因子：
--
作者：
Lawn SD;Mwaba P;Bates M;Piatek A;Alexander H;Marais BJ;Cuevas LE;McHugh TD;Zijenah L;Kapata N;Abubakar I;McNerney R;Hoelscher M;Memish ZA;Migliori GB;Kim P;Maeurer M;Schito M;Zumla A
通讯作者：
Zumla A
影响因子：
13.2
作者：
Engel, Gregory A.;Wilbur, Alicia K.;Jones-Engel, Lisa
通讯作者：
Jones-Engel, Lisa
影响因子：
4.4
作者：
Falkinham, JO;Norton, CD;LeChevallier, MW
通讯作者：
LeChevallier, MW
影响因子：
2.4
作者：
Wilbur, Alicia K.;Engel, Gregory A.;Jones-Engel, Lisa
通讯作者：
Jones-Engel, Lisa
影响因子：
2.2
作者：
Savelkoul, Paul H. M.;Catsburg, Arnold;Noordhoek, Gerda T.
通讯作者：
Noordhoek, Gerda T.