Coproscopical diagnosis of patent Fasciola hepatica infections in sheep - A comparison between standard sedimentation, FLUKEFINDER® and a combination of both.
Coproscopical diagnosis of patent Fasciola hepatica infections in sheep - A comparison between standard sedimentation, FLUKEFINDER® and a combination of both.
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绵羊肝片形吸虫感染的腹腔镜诊断 - 标准沉降法、FLUKEFINDER® 以及两者组合之间的比较。
DOI：
10.1016/j.vetpar.2023.109956
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发表时间：
2023
影响因子：
2.6
通讯作者：
Kahl A
中科院分区：
文献类型：
--
作者：
Kahl A
肝片形吸虫是一种高致病性的人畜共患吸虫，广泛分布于世界各地。在牲畜中，如果不及时诊断和有效治疗，感染可能导致重大经济损失。特别是对于小型反刍动物，检测吸虫感染的标准方法是基于粪便检查方法，如沉降法，其检测粪便样品中的肝片吸虫卵。在这方面，最近诊断专利感染的创新性粪便检查方法是FLUKEFINDER®方法，其依赖于沉降前的差异筛分。比较了这两种方法以及允许用FLUKEFINDER®装置处理更大量粪便的两种方法的组合，以评估哪种方法最适合确定F的患病率和强度。肝虫卵脱落这些方法进行了比较，他们的能力，以恢复鸡蛋从绵羊粪便样品含有不同数量的吸虫卵每克（EPG）的粪便和稀释的样品进一步与未感染的绵羊粪便混合。为了比较检测方法的特异性，由对样本性质不知情的研究者平行分析EPG较低的阳性和阴性样本。发现关于EPG结果的显著差异：FLUKEFINDER®方法在未稀释样品以及所有混合水平中显示出最高EPG值（p < 0.001），其次是改良的FLUKEFINDER®方法。标准沉降显示最低EPG值和技术重复之间的最高变异性。FLUKEFINDER®方法和改进的FLUKEFINDER®方法的精度显著高于标准沉降的精度，如通过比较技术重复之间的变异性所确定的。使用改进的FLUKEFINDER®方法检测到最高的生鸡蛋计数。即使在最低的蛋浓度下，FLUKEFINDER®方法和组合方法也显示出100%的灵敏度，而对于同一组样品（即一个假阴性样品），标准沉降的灵敏度为98.1%。在一项旨在估计特异性的单独研究中，未发现三种方法之间存在差异：所有方案均显示100%特异性，并且能够正确区分真正阳性和真正阴性样本，而没有任何证据表明平行处理的阳性和阴性样本之间存在交叉污染。
The liver fluke Fasciola hepatica is a highly pathogenic and zoonotic trematode with a cosmopolitan distribution. In livestock, infections may lead to significant economic losses if not diagnosed promptly and treated effectively. Particularly for small ruminants, the standard method for the detection of fluke infection is based on coproscopical methods such as the sedimentation method, which detectsF.hepaticaeggs in faecal samples. In this respect a recent innovative coproscopical approach to diagnose patent infections is the FLUKEFINDER® method, which relies on differential sieving before sedimentation. These two methods and a combination of both methods that allows larger amounts of faeces to be processed with the FLUKEFINDER® apparatus were compared, to assess which method is most appropriate to determine the prevalence and intensity ofF. hepaticaegg shedding. The methods were compared for their ability to recover eggs from ovine faecal samples containing different numbers of fluke eggs per gram (EPG) of faeces and diluting the samples further by mixing with faeces from uninfected sheep. To compare the specificity of the test procedures, positive and negative samples with a low EPG were analysed in parallel by an investigator blinded to the nature of the samples. Significant differences concerning the EPG outcome were found: The FLUKEFINDER® method demonstrated the highest EPG values (p < 0.001) in the undiluted samples as well as in all mixing levels, followed by the modified FLUKEFINDER® method. The standard sedimentation showed the lowest EPG values and the highest variability between technical replicates. The precision of the FLUKEFINDER® method and the modified FLUKEFINDER® method were significantly higher than the precision of the standard sedimentation as determined by comparison of variability between technical replicates. The highest raw egg counts were detected using the modified FLUKEFINDER® method. The FLUKEFINDER® method and the combined method showed a sensitivity of 100 % even at the lowest egg concentrations, whereas the sensitivity of the standard sedimentation was 98.1 % for the same set of samples (i.e. one false negative sample). In a separate investigation aiming to estimate the specificity no differences were found between the three methods: all protocols showed 100 % specificity and were able to correctly distinguish between truly positive and truly negative samples without any evidence of cross-contamination between positive and negative samples processed in parallel.
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影响因子：
2.6
作者：
P. Torgerson;M. Paul;F. Lewis
通讯作者：
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卡哈马卡（秘鲁）牛肝片吸虫对三氯苯达唑的耐药性：绵羊的临床试验和体内功效测试。
影响因子：
2.6
作者：
P. Ortiz;S. Scarcella;C. Cerna;C. Rosales;M. Cabrera;M. Guzmán;P. Lamenza;H. Solana
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H. Solana
肝吸虫感染对牛羊生产影响的荟萃分析
DOI：
10.1101/2020.07.29.227074
发表时间：
2020
期刊：
--
影响因子：
--
作者：
Hayward A
通讯作者：
Hayward A
影响因子：
2.2
作者：
Sargison, N. D.;Scott, P. R.
通讯作者：
Scott, P. R.
DOI：
--
发表时间：
2017
期刊：
影响因子：
--
作者：
A. Forbes
通讯作者：
A. Forbes