The viscosity behaviors of bacterial suspensions or extracellular polymeric substances and their effects on aerobic granular sludge

The viscosity behaviors of bacterial suspensions or extracellular polymeric substances and their effects on aerobic granular sludge
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细菌悬浮液或胞外聚合物的粘度行为及其对好氧颗粒污泥的影响

DOI:
10.1007/s11356-019-06012-1
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发表时间:
2019-08
影响因子:
5.8
通讯作者:
Zhu Jianrong
Zhu Jianrong
中科院分区:
环境科学与生态学3区
文献类型:
--
作者:
Yang Xilan;Xu Tianguang;Cao Pei;Qiao Kai;Wang Lei;Zhao Tingting;Zhu Jianrong

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虽然细菌和胞外聚合物(EPS)在絮凝活性污泥(FAS)和好氧颗粒污泥(AGS)中的粘度行为已经被研究,但在纯培养中,还没有关于粘度在微生物附着中的作用的研究。本研究考察了细菌和EPS的粘度行为。结果表明,细菌及其EPS表现出非牛顿流体和剪切稀化行为。细菌黏度为1.55~3.80cp,胞外多糖黏度为1.10~2.40cp,细菌附着率为0.1426~3.1015。附着力高的细菌分泌粘度较高的EPS(2.40个CP),而附着较弱的细菌分泌粘度较低的EPS(1.10个CP)。黏度与微生物附着或胞外多糖含量呈显著正相关。PS含量是细菌黏度的来源,β-多糖对黏度和微生物附着的影响比α-多糖更大。因此,粘度对微生物附着起着至关重要的作用,较高的粘度和PS含量会导致较高的微生物附着,这有利于AGS的造粒过程。
Although the viscosity behavior of bacteria and extracellular polymeric substances (EPS) in flocculent activated sludge (FAS) and aerobic granular sludge (AGS) has been investigated, no studies have explored the role of viscosity in microbial attachment in pure culture. This study investigated the viscosity behavior of bacteria and EPS. The results showed that bacteria and their EPS exhibited non-Newtonian fluid and shear-thinning behavior. The viscosity of bacteria and EPS was 1.55–3.80 cP and 1.10–2.40 cP, respectively, while the attachment of bacteria (optical density at 600 nm) was 0.1426–3.1015. Bacteria with high attachment secreted EPS with a higher viscosity (2.40 cP), whereas those with weak attachment expressed EPS with a lower viscosity (1.10 cP). Viscosity and microbial attachment or extracellular polysaccharide (PS) content were significantly positively correlated. PS content was the source of bacterial viscosity, and β-polysaccharide played a more important role in viscosity and microbial attachment than α-polysaccharide. Thus, viscosity plays a critical role in microbial attachment, and high viscosity and PS content result in high microbial attachment, which is beneficial to the granulation process of AGS.
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