The effect of short-term exercise prehabilitation on skeletal muscle protein synthesis and atrophy during bed rest in older men.
The effect of short-term exercise prehabilitation on skeletal muscle protein synthesis and atrophy during bed rest in older men.
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DOI：
10.1002/jcsm.12661
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Breen L
中科院分区：
文献类型：
--
作者：
Smeuninx B;Elhassan YS;Manolopoulos KN;Sapey E;Rushton AB;Edwards SJ;Morgan PT;Philp A;Brook MS;Gharahdaghi N;Smith K;Atherton PJ;Breen L
停用期恢复不良会加速与年龄相关的肌肉损失，使个体容易出现继发性不良健康后果。停用前的锻炼（预康复）可以防止随后卸载期间的肌肉退化。本研究旨在调查老年男性卧床休息 5 天期间短期阻力运动训练 (RET) 预康复对肌肉形态和调节机制的影响。 10 名 65-80 岁的健康老年男性在住院卧床休息 5 天之前，在 7 天内接受了四次高强度单侧腿部 RET 训练。在 RET 预康复和卧床休息过程中监测身体活动和步数，同时记录饮食摄入量。在卧床休息之前和之后，测定股四头肌横截面积（CSA）和激素/脂质分布。使用连续肌肉活检和双稳定同位素示踪剂来确定 RET 预康复和卧床阶段的综合肌原纤维蛋白合成 (iMyoPS)，以及卧床休息结束时的急性吸收后和餐后肌原纤维蛋白合成 (aMyoPS) 率。卧床休息期间，与习惯水平相比，每日步数以及轻度和中度体力活动时间减少，而久坐时间增加（全部 P < 0.001）。卧床休息期间的膳食蛋白质和纤维摄入量低于习惯值（两者 P < 0.01）。训练后的腿 (EX) 的 iMyoPS 率显着高于未运动的对照腿 (CTL)（分别为 1.76 ± 0.37%/天和 1.36 ± 0.18%/天；P = 0.007）。 EX 和 CTL 卧床休息期间 iMyoPS 率下降类似（CTL，1.07 ± 0.22%/天；EX，1.30 ± 0.38%/天；P 分别 = 0.037 和 0.002）。餐后 aMyoPS 率仅在 EX 中高于吸收后值 (P = 0.018)，双腿之间的餐后 aMyoPS 刺激增量没有差异。与卧床休息相比，EX 和 CTL 中股四头肌 CSA 的 40%、60% 和 80% 肌肉长度显着减少（分别为 0.69%、3.5% 和 2.8%；全部 P < 0.01），双腿之间没有差异。在腿部之间或卧床休息时，没有观察到纤维型 CSA 的差异。血浆胰岛素和血脂不随卧床休息而改变。短期抗阻运动预康复增加了老年男性的 iMyoPS 发生率，但并没有抵消卧床休息期间 iMyoPS 和肌肉质量的相对下降。
Poor recovery from periods of disuse accelerates age‐related muscle loss, predisposing individuals to the development of secondary adverse health outcomes. Exercise prior to disuse (prehabilitation) may prevent muscle deterioration during subsequent unloading. The present study aimed to investigate the effect of short‐term resistance exercise training (RET) prehabilitation on muscle morphology and regulatory mechanisms during 5 days of bed rest in older men. Ten healthy older men aged 65–80 years underwent four bouts of high‐volume unilateral leg RET over 7 days prior to 5 days of inpatient bed rest. Physical activity and step‐count were monitored over the course of RET prehabilitation and bed rest, whilst dietary intake was recorded throughout. Prior to and following bed rest, quadriceps cross‐sectional area (CSA), and hormone/lipid profiles were determined. Serial muscle biopsies and dual‐stable isotope tracers were used to determine integrated myofibrillar protein synthesis (iMyoPS) over RET prehabilitation and bed rest phases, and acute postabsorptive and postprandial myofibrillar protein synthesis (aMyoPS) rates at the end of bed rest. During bed rest, daily step‐count and light and moderate physical activity time decreased, whilst sedentary time increased when compared with habitual levels (P < 0.001 for all). Dietary protein and fibre intake during bed rest were lower than habitual values (P < 0.01 for both). iMyoPS rates were significantly greater in the exercised leg (EX) compared with the non‐exercised control leg (CTL) over prehabilitation (1.76 ± 0.37%/day vs. 1.36 ± 0.18%/day, respectively; P = 0.007). iMyoPS rates decreased similarly in EX and CTL during bed rest (CTL, 1.07 ± 0.22%/day; EX, 1.30 ± 0.38%/day; P = 0.037 and 0.002, respectively). Postprandial aMyoPS rates increased above postabsorptive values in EX only (P = 0.018), with no difference in delta postprandial aMyoPS stimulation between legs. Quadriceps CSA at 40%, 60%, and 80% of muscle length decreased significantly in EX and CTL over bed rest (0.69%, 3.5%, and 2.8%, respectively; P < 0.01 for all), with no differences between legs. No differences in fibre‐type CSA were observed between legs or with bed rest. Plasma insulin and serum lipids did not change with bed rest. Short‐term resistance exercise prehabilitation augmented iMyoPS rates in older men but did not offset the relative decline in iMyoPS and muscle mass during bed rest.
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影响因子：
4.2
作者：
Ethgen O;Beaudart C;Buckinx F;Bruyère O;Reginster JY
通讯作者：
Reginster JY
影响因子：
5.8
作者：
Blanc, S;Normand, S;Gauquelin-Koch, G
通讯作者：
Gauquelin-Koch, G
影响因子：
5.1
作者：
Aggio, Daniel A.;Sartini, Claudio;Jefferis, Barbara J.
通讯作者：
Jefferis, Barbara J.
影响因子：
5.5
作者：
Damas, Felipe;Phillips, Stuart M.;Ugrinowitsch, Carlos
通讯作者：
Ugrinowitsch, Carlos
影响因子：
3.4
作者：
Buckley, John P.;Borg, Gunnar A. V.
通讯作者：
Borg, Gunnar A. V.