A microsatellite-based linkage map of salt tolerant tilapia (Oreochromis mossambicus x Oreochromis spp.) and mapping of sex-determining loci.
A microsatellite-based linkage map of salt tolerant tilapia (Oreochromis mossambicus x Oreochromis spp.) and mapping of sex-determining loci.
复制标题
DOI：
10.1186/1471-2164-14-58
复制
发表时间：
2013-01-28
期刊：
影响因子：
4.4
通讯作者：
Yue GH
中科院分区：
文献类型：
--
作者：
Liu F;Sun F;Li J;Xia JH;Lin G;Tu RJ;Yue GH
罗非鱼是一组罗非鱼的通称，是最重要的水产养殖食用鱼之一。莫桑比克罗非鱼及其杂交种，包括红罗非鱼，是耐盐罗非鱼的主要代表。连锁图谱是定位重要性状的QTL、基因定位克隆和了解基因组进化的基本框架。我们利用来自两个F1家系的95个个体和包括282个EST标记在内的401个微卫星构建了莫桑比克罗非鱼和红罗非鱼的共识连锁图谱。此外，我们还进行了比较定位，并在全基因组上寻找性别决定基因座。这401个微卫星被划分为22个连锁群。该图谱全长1067.6 cM，平均标记间距离为3.33 cM。罗非鱼和刺鱼、青鱼、河豚和斑马鱼的比较图谱显示了不同物种的染色体之间明显的同源关系。我们发现了两组两个独立的染色体融合形成两个新的染色体对的证据，导致其祖先的24对染色体减少到罗非鱼的22对。将莫桑比克罗非鱼的XY性别决定基因定位在LG1上，并在5个家系549个个体中进行了验证。红罗非鱼的XY性别决定基因主要位于LG22上，并在两个家系275个个体中进行了验证。利用401个微卫星构建了耐盐罗非鱼第一代连锁图谱。两组两个独立的染色体的两次单独融合可能导致其祖先的24对染色体减少到罗非鱼的22对。将莫桑比克罗非鱼和红罗非鱼的XY性别决定基因座分别定位在LG1和LG22上。该图谱为重要性状的QTL定位和比较基因组研究提供了有用的资源。与性别决定基因连锁的DNA标记可用于选育耐盐罗非鱼全雄群体的YY雄鱼，也可用于鱼类性别决定机制的研究。
Tilapia is the common name for a group of cichlid fishes and is one of the most important aquacultured freshwater food fish. Mozambique tilapia and its hybrids, including red tilapia are main representatives of salt tolerant tilapias. A linkage map is an essential framework for mapping QTL for important traits, positional cloning of genes and understanding of genome evolution. We constructed a consensus linkage map of Mozambique tilapia and red tilapia using 95 individuals from two F1 families and 401 microsatellites including 282 EST-derived markers. In addition, we conducted comparative mapping and searched for sex-determining loci on the whole genome. These 401 microsatellites were assigned to 22 linkage groups. The map spanned 1067.6 cM with an average inter-marker distance of 3.3 cM. Comparative mapping between tilapia and stickleback, medaka, pufferfish and zebrafish revealed clear homologous relationships between chromosomes from different species. We found evidence for the fusion of two sets of two independent chromosomes forming two new chromosome pairs, leading to a reduction of 24 chromosome pairs in their ancestor to 22 pairs in tilapias. The XY sex determination locus in Mozambique tilapia was mapped on LG1, and verified in five families containing 549 individuals. The major XY sex determination locus in red tilapia was located on LG22, and verified in two families containing 275 individuals. A first-generation linkage map of salt tolerant tilapia was constructed using 401 microsatellites. Two separate fusions of two sets of two independent chromosomes may lead to a reduction of 24 chromosome pairs in their ancestor to 22 pairs in tilapias. The XY sex-determining loci from Mozambique tilapia and red tilapia were mapped on LG1 and LG22, respectively. This map provides a useful resource for QTL mapping for important traits and comparative genome studies. The DNA markers linked to the sex-determining loci could be used in the selection of YY males for breeding all-male populations of salt tolerant tilapia, as well as in studies on mechanisms of sex determination in fish.
登录
查看更多内容
影响因子：
1.6
作者：
Chew, JSK;Oliveira, C;Dobson, MJ
通讯作者：
Dobson, MJ
影响因子：
4.4
作者：
Guyomard, Rene;Mauger, Stephane;Tabet-Canale, Kamila;Martineau, Sylvain;Genet, Carine;Krieg, Francine;Quillet, Edwige
通讯作者：
Quillet, Edwige
影响因子：
4.4
作者：
Feldmeyer B;Wheat CW;Krezdorn N;Rotter B;Pfenninger M
通讯作者：
Pfenninger M
影响因子：
4.5
作者：
Agresti, JJ;Seki, S;May, B
通讯作者：
May, B
影响因子：
5.8
作者：
Kofler, Robert;Schloetterer, Christian;Lelley, Tamas
通讯作者：
Lelley, Tamas