How do you build a nectar spur? A transcriptomic comparison of nectar spur development in Linaria vulgaris and gibba development in Antirrhinum majus.
How do you build a nectar spur? A transcriptomic comparison of nectar spur development in Linaria vulgaris and gibba development in Antirrhinum majus.
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DOI：
10.3389/fpls.2023.1190373
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发表时间：
2023
影响因子：
5.6
通讯作者：
Glover, Beverley J.
中科院分区：
文献类型：
--
作者：
Cullen, Erin;Wang, Qi;Glover, Beverley J.
花蜜刺(花器官的管状突起)长期以来一直令生物学家着迷。然而，鉴于没有模式物种拥有花蜜刺状物，关于它们的发育仍有许多需要了解的地方。在这项研究中，我们结合了形态分析和比较转录组学，以获得一个全局洞察刺突生长的形态和分子基础。对两个相关物种在三个关键发育阶段(通过我们的形态分析鉴定)进行了全转录组测序，其中一个物种有突起(Linaria Uggaris)，一个没有突起(金鱼草)。我们选择了一系列短刺特异基因，并对其进行了基因富集化分析。我们的RNA-seq分析结果与我们的形态观察结果一致。我们描述了短刺发育过程中的基因活动，并提供了短刺特异基因的目录。我们的短枝特异基因清单丰富了与植物激素细胞分裂素、生长素和赤霉素相关的基因。我们介绍了与普通乳杆菌刺激性发育相关的基因的全球视角，并定义了一套特定于刺激性发育的基因。这项工作为普通乳杆菌的生长发育刺激提供了候选基因，可以在未来的研究中对其进行研究。
Nectar spurs (tubular outgrowths of floral organs) have long fascinated biologists. However, given that no model species possess nectar spurs, there is still much to learn about their development. In this study we combined morphological analysis with comparative transcriptomics to gain a global insight into the morphological and molecular basis of spur outgrowth in Linaria. Whole transcriptome sequencing was performed on two related species at three key developmental stages (identified by our morphological analysis), one with a spur (Linaria vulgaris), and one without a spur (Antirrhinum majus). A list of spur-specific genes was selected, on which we performed a gene enrichment analysis. Results from our RNA-seq analysis agreed with our morphological observations. We describe gene activity during spur development and provide a catalogue of spur-specific genes. Our list of spur-specific genes was enriched for genes connected to the plant hormones cytokinin, auxin and gibberellin. We present a global view of the genes involved in spur development in L. vulgaris, and define a suite of genes which are specific to spur development. This work provides candidate genes for spur outgrowth and development in L. vulgaris which can be investigated in future studies.
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