Analysis of the role of Arabidopsis class I TCP genes AtTCP7, AtTCP8, AtTCP22, and AtTCP23 in leaf development.
Analysis of the role of Arabidopsis class I TCP genes AtTCP7, AtTCP8, AtTCP22, and AtTCP23 in leaf development.
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DOI：
10.3389/fpls.2013.00406
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发表时间：
2013
影响因子：
5.6
通讯作者：
Sinha N
中科院分区：
文献类型：
--
作者：
Aguilar-Martínez JA;Sinha N
植物特异性转录因子TCP家族通过控制细胞增殖和分化来调节植物形态。该基因家族由两组组成，即I类和II类。虽然II类TCP基因在植物发育中的作用已广为人知，但一些I类TCP基因的功能数据却很缺乏。我们研究了一组系统发育相关的I类TCP基因：AtTCP7、AtTCP8、AtTCP22和AtTCP23。在生长的幼叶中发现该组基因具有相似的表达模式，这表明基因功能具有相似性。基因冗余是该组基因的特征，在II类TCP基因中也可见到这种情况。我们构建了一个五重突变体tcp8 tcp15 tcp21 tcp22 tcp23，并表明这些基因功能的缺失会导致叶片发育性状的改变。我们还确定这些因子能够在酵母双杂交试验中相互作用，并调节KNOX1基因的表达。为了规避基因冗余问题，使用了带有SRDX抑制结构域的显性负性形式。对表达AtTCP7 - SRDX和AtTCP23 - SRDX的转基因植物的分析表明，这些因子在控制细胞增殖方面具有作用。
TCP family of plant-specific transcription factors regulates plant form through control of cell proliferation and differentiation. This gene family is comprised of two groups, class I and class II. While the role of class II TCP genes in plant development is well known, data about the function of some class I TCP genes is lacking. We studied a group of phylogenetically related class I TCP genes: AtTCP7, AtTCP8, AtTCP22, and AtTCP23. The similar expression pattern in young growing leaves found for this group suggests similarity in gene function. Gene redundancy is characteristic in this group, as also seen in the class II TCP genes. We generated a pentuple mutant tcp8 tcp15 tcp21 tcp22 tcp23 and show that loss of function of these genes results in changes in leaf developmental traits. We also determined that these factors are able to mutually interact in a yeast two-hybrid assay and regulate the expression of KNOX1 genes. To circumvent the issue of genetic redundancy, dominant negative forms with SRDX repressor domain were used. Analysis of transgenic plants expressing AtTCP7-SRDX and AtTCP23-SRDX indicate a role of these factors in the control of cell proliferation.
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影响因子：
11.6
作者：
Guo, Mengjuan;Thomas, Julie;Timmermans, Marja C. P.
通讯作者：
Timmermans, Marja C. P.
DOI：
10.1007/bf00331014
发表时间：
1986-01-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
KONCZ, C;SCHELL, J
通讯作者：
SCHELL, J
影响因子：
64.8
作者：
Doebley, J;Stec, A;Hubbard, L
通讯作者：
Hubbard, L
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
7.4
作者：
Danisman, Selahattin;van der Wal, Froukje;Immink, Richard G. H.
通讯作者：
Immink, Richard G. H.