The kinetic landscape and interplay of protein networks in cytokinesis.
The kinetic landscape and interplay of protein networks in cytokinesis.
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DOI：
10.1016/j.isci.2020.101917
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发表时间：
2021-01-22
期刊：
影响因子：
5.8
通讯作者：
Bi E
中科院分区：
文献类型：
--
作者：
Okada H;MacTaggart B;Ohya Y;Bi E
胞质分裂是由组织成功能模块的蛋白质网络执行的。每个模块中的单个蛋白质已被不同程度地表征。然而，模块的集体行为和相互作用仍然知之甚少。在这项研究中，我们进行了定量的时间推移成像分析的积累动力学的20多个蛋白质从不同模块的胞质分裂在芽殖酵母。这种分析导致了全面的图片的动力学景观的胞质分裂，从肌动球蛋白环（AMR）的组装细胞分离。这表明AMR经历双相收缩，并且收缩阶段之间的转换可能是由AMR成熟和初级隔膜形成触发的。该分析还提供了对肌动蛋白丝和转氨酶样蛋白Cyk 3在胞质分裂中的功能的进一步了解，此外，将Kre 6定义为催化β-1，6-葡聚糖合成以在细胞生长和分裂期间驱动细胞壁成熟的可能酶。胞质分裂由蛋白质模块执行，每个模块具有独特的动力学特征肌动球蛋白环以精心调节的双相方式收缩Cyk 3中的转氨酶样结构域在胞质分裂中起双重作用Kre 6在生长和分裂期间催化细胞表面的β-1，6-葡聚糖合成生物科学;分子生物学;细胞生物学
Cytokinesis is executed by protein networks organized into functional modules. Individual proteins within each module have been characterized to various degrees. However, the collective behavior and interplay of the modules remain poorly understood. In this study, we conducted quantitative time-lapse imaging to analyze the accumulation kinetics of more than 20 proteins from different modules of cytokinesis in budding yeast. This analysis has led to a comprehensive picture of the kinetic landscape of cytokinesis, from actomyosin ring (AMR) assembly to cell separation. It revealed that the AMR undergoes biphasic constriction and that the switch between the constriction phases is likely triggered by AMR maturation and primary septum formation. This analysis also provided further insights into the functions of actin filaments and the transglutaminase-like protein Cyk3 in cytokinesis and, in addition, defined Kre6 as the likely enzyme that catalyzes β-1,6-glucan synthesis to drive cell wall maturation during cell growth and division. Cytokinesis is executed by protein modules each with a unique kinetic signature Actomyosin ring constricts in a biphasic manner that is elaborately regulated The transglutaminase-like domain in Cyk3 plays a dual role in cytokinesis Kre6 catalyzes β-1,6-glucan synthesis at the cell surface during growth and division Biological Sciences; Molecular Biology; Cell Biology
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