Analysis of the in vitro degradation and the in vivo tissue response to bi-layered 3D-printed scaffolds combining PLA and biphasic PLA/bioglass components - Guidance of the inflammatory response as basis for osteochondral regeneration.
Analysis of the in vitro degradation and the in vivo tissue response to bi-layered 3D-printed scaffolds combining PLA and biphasic PLA/bioglass components - Guidance of the inflammatory response as basis for osteochondral regeneration.
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DOI：
10.1016/j.bioactmat.2017.06.001
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发表时间：
2017-12
影响因子：
18.9
通讯作者：
Ghanaati S
中科院分区：
文献类型：
--
作者：
Barbeck M;Serra T;Booms P;Stojanovic S;Najman S;Engel E;Sader R;Kirkpatrick CJ;Navarro M;Ghanaati S
本研究的目的是体外和体内分析复合聚乳酸和双相聚乳酸/生物玻璃G5层的双层3D打印支架在体内再生骨软骨缺损的作用。体外分析的重点是在SBF中浸泡后的(分子)重量损失和形态和力学变化。体内研究的重点是利用CD-1小鼠建立的皮下植入模型分析种植床血管形成的组织反应和差异，并建立组织学和组织形态计量学方法。两种支架均保持了其结构的完整性，但形态发生了变化，其中以聚乳酸/G5支架最为明显。材料的力学性能随降解程度的增加而降低，而聚乳酸/G5支架的压缩弹性系数高于聚乳酸支架。对聚乳酸的组织反应表现为骨髓间充质干细胞数量较少，种植床血管化程度较低，而加入G5后，骨髓间充质细胞数量较多，种植床血管形成较多。分析表明，使用双层支架显示了在体内观察不同反应的能力，尽管解放军和解放军/G5层在物理上很接近。综上所述，结果表明，G5的加入能够减少支架的重量损失，提高机械强度。此外，G5的加入导致了种植床骨组织再生所需的更高的血管化，而在聚乳酸部分，骨组织再生所需的骨组织再生所需的血管化明显减少，而对于软骨再生，则发现显著较低的血管形成。因此，这一数据表明，所分析的双层支架可以作为骨软骨组织缺损再生的理想基础。此外，结果表明，它可能能够减少所需的实验动物数量，因为它可能分析一个实验动物中多个植入物的组织反应。分析了一种新型的骨软骨组织再生方法--聚乳酸和聚乳酸/G5生物玻璃双层支架。体外降解分析表明，材料的形态发生了变化，特别是聚乳酸/G5支架。聚乳酸/G5支架具有较高的压缩模量值，证实了G5的增强效果。聚乳酸诱导单核细胞和低血管形成，生物玻璃诱导多核巨细胞和更高的血管形成。G5生物玻璃促进骨再生，而聚乳酸支架基于细胞介导的血管形成促进软骨再生。
The aim of the present study was the in vitro and in vivo analysis of a bi-layered 3D-printed scaffold combining a PLA layer and a biphasic PLA/bioglass G5 layer for regeneration of osteochondral defects in vivo Focus of the in vitro analysis was on the (molecular) weight loss and the morphological and mechanical variations after immersion in SBF. The in vivo study focused on analysis of the tissue reactions and differences in the implant bed vascularization using an established subcutaneous implantation model in CD-1 mice and established histological and histomorphometrical methods. Both scaffold parts kept their structural integrity, while changes in morphology were observed, especially for the PLA/G5 scaffold. Mechanical properties decreased with progressive degradation, while the PLA/G5 scaffolds presented higher compressive modulus than PLA scaffolds. The tissue reaction to PLA included low numbers of BMGCs and minimal vascularization of its implant beds, while the addition of G5 lead to higher numbers of BMGCs and a higher implant bed vascularization. Analysis revealed that the use of a bi-layered scaffold shows the ability to observe distinct in vivo response despite the physical proximity of PLA and PLA/G5 layers. Altogether, the results showed that the addition of G5 enables to reduce scaffold weight loss and to increase mechanical strength. Furthermore, the addition of G5 lead to a higher vascularization of the implant bed required as basis for bone tissue regeneration mediated by higher numbers of BMGCs, while within the PLA parts a significantly lower vascularization was found optimally for chondral regeneration. Thus, this data show that the analyzed bi-layered scaffold may serve as an ideal basis for the regeneration of osteochondral tissue defects. Additionally, the results show that it might be able to reduce the number of experimental animals required as it may be possible to analyze the tissue response to more than one implant in one experimental animal. A bi-layered scaffold (PLA and PLA/G5 bioglass) as a novel approach for osteochondral tissue regeneration has been analyzed. An in vitro degradation analysis showed changes in morphology, especially for the PLA/G5 scaffold. PLA/G5 scaffolds presented higher compressive modulus confirming the reinforcing effect of G5. PLA induced mononucleated cells and a low vascularization, while bioglass induced multinucleated giant cells and a higher vascularization. G5 bioglass promotes bone regeneration, while the PLA scaffolds promote chondral regeneration based on cell-mediated vascularization.
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DOI：
10.1016/j.bbrc.2010.01.109
发表时间：
2010-02-26
影响因子：
3.1
作者：
Aguirre, A.;Gonzalez, A.;Engel, E.
通讯作者：
Engel, E.
影响因子：
3.3
作者：
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影响因子：
4
作者：
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DOI：
10.1016/0030-4220(71)90044-2
发表时间：
1971-01-01
影响因子：
--
作者：
CUTRIGHT, DE;HUNSUCK, EE
通讯作者：
HUNSUCK, EE
影响因子：
1.6
作者：
Barbeck, Mike;Udeabor, Samuel;Ghanaati, Shahram
通讯作者：
Ghanaati, Shahram