Expression of Proteasome Subunits Low Molecular Mass Polypeptide (LMP) 2 and LMP7 in the Endometrium and Placenta of Rhesus Monkey (Macaca mulatta) During Early Pregnancy1-猫眼课题宝

妊娠早期恒河猴子宫内膜和胎盘中蛋白酶体亚基低分子量多肽（LMP）2和LMP7的表达1

DOI：

发表时间：

2004

期刊：

Biology of Reproduction

影响因子：

3.6

通讯作者：

中科院分区：

生物学2区

文献类型：

作者：

Hong;Hong;Q. Li;Hai;Dong;Cheng Zhu

摘要已有研究表明，泛素-蛋白酶体途径在胚胎着床过程中起着重要作用。低分子量多肽（LMP）2和LMP 7是20 S蛋白酶体的两个亚基，对蛋白酶体的活性至关重要。为了进一步阐明LMP 2和LMP 7在妊娠早期胚胎着床中的作用，我们克隆了LMP 2和LMP 7基因的部分序列，并研究了LMP 2和LMP 7在恒河猴（Macaca mulatta）妊娠第12、18和26天子宫中的时空表达。结果表明，349-bp的LMP 2片段和340-bp的LMP 7片段与人类同源物的同源性分别为97%和99%。从逆转录-聚合酶链反应、原位杂交和免疫组化的统计结果来看，我们发现LMP 2和LMP 7的表达水平随着妊娠时间的延长而显著增加。LMP 2和LMP 7 mRNA在妊娠第12天主要表达于子宫腔上皮和腺上皮。在妊娠第18天和第26天，在胎盘绒毛、滋养层柱和靠近着床部位的动脉内皮细胞中检测到LMP 2和LMP 7 mRNA的强信号，在滋养层外壳和腺上皮中发现中度表达。LMP 2和LMP 7 mRNA在妊娠第26天广泛分布于间质中。LMP 2和LMP 7蛋白的表达模式与其转录本相似，但在妊娠的各个阶段的间质中检测到LMP 2和LMP 7蛋白的弱免疫染色。这些结果表明，LMP 2和LMP 7可能参与滋养层细胞侵袭，血管生成，细胞外基质降解，免疫耐受和腺体分泌的一些关键过程。

Abstract Previous studies have demonstrated that the ubiquitin-proteasome pathway plays an important role in embryo implantation. Low molecular mass polypeptide (LMP) 2 and LMP7 are the two subunits of 20S proteasome, which are critical for proteasome activity. To further elucidate the roles of LMP2 and LMP7 in embryo implantation during early pregnancy, we cloned partial sequences of the LMP2 and LMP7 genes and studied the spatiotemporal expression of LMP2 and LMP7 in rhesus monkey (Macaca mulatta) uteri on Days 12, 18, and 26 of pregnancy. The results showed that the 349-base pair (bp) LMP2 fragment and the 340-bp LMP7 fragment were 97% and 99% identical, respectively, to those of human homologues. From the statistical results of reverse transcription-polymerase chain reaction, in situ hybridization, and immunohistochemistry, we found that the expression levels of LMP2 and LMP7 significantly increased with the elongation of pregnancy. The LMP2 and LMP7 mRNAs were mainly expressed in the luminal and glandular epithelium on Day 12 of pregnancy. On Days 18 and 26 of pregnancy, strong signals of LMP2 and LMP7 mRNAs were detected in the placental villi, trophoblastic column, and arterial endothelial cells close to the implantation site, and moderate expressions were found in the trophoblastic shell and glandular epithelium. The LMP2 and LMP7 mRNAs were extensively distributed in the stroma on Day 26 of pregnancy. The expression patterns of LMP2 and LMP7 proteins were similar to those of their transcripts, but weak immunostaining of LMP2 and LMP7 proteins was detected in stroma at all stages of pregnancy. These results suggest that LMP2 and LMP7 may be involved in some key processes of trophoblastic invasion, angiogenesis, degradation of the extracellular matrix, immune tolerance, and glandular secretion.