Isolation and analyses of lamina propria lymphocytes from mouse intestines.
Isolation and analyses of lamina propria lymphocytes from mouse intestines.
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从小鼠肠中分离和分析固定层淋巴细胞。
DOI：
10.1016/j.xpro.2022.101366
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发表时间：
2022-06-17
期刊：
影响因子：
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通讯作者：
Huh, Jun R.
中科院分区：
文献类型：
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作者：
Kim, Eunha;Tran, Melissa;Sun, Yanyi;Huh, Jun R.
研究肠道免疫反应是了解局部和全身免疫的关键。然而，获得具有高细胞活力的常驻肠道免疫细胞可能具有挑战性。在这里，我们提供了一个优化的方案，从小肠和大肠分离固有层淋巴细胞，包括淋巴细胞活化细胞因子表达分析和表面和细胞内抗体染色和流式细胞术技术。该方案可用于分离和分析组织驻留免疫细胞从其他组织与指定修饰。有关使用和执行本协议的完整详情，请参阅。小鼠肠道固有层淋巴细胞分离和分析的优化方案分离细胞的刺激细胞内细胞因子分析通过抗体染色和流式细胞术评估T和B细胞亚型组成研究肠道免疫应答对了解局部和全身免疫至关重要。然而，获得具有高细胞活力的常驻肠道免疫细胞可能具有挑战性。在这里，我们提供了一个优化的方案，从小肠和大肠分离固有层淋巴细胞，包括淋巴细胞活化细胞因子表达分析和表面和细胞内抗体染色和流式细胞术技术。该方案可用于分离和分析组织驻留免疫细胞从其他组织与指定修饰。
Investigating intestinal immune responses is critical to understanding local and systemic immunity. However, obtaining resident intestinal immune cells with high cell viability can be challenging. Here, we provide an optimized protocol to isolate lamina propria lymphocytes from the small and large intestines, including lymphocyte activation for cytokine expression analysis and techniques for surface and intracellular antibody staining and flow cytometry. This protocol can be used for isolating and analyzing tissue-resident immune cells from other tissues with specified modifications. For complete details on the use and execution of this protocol, please refer to. Optimized protocol to isolate and analyze mouse intestinal lamina propria lymphocytes Stimulation of isolated cells for intracellular cytokine analysis Assessment of T and B cell subtype composition via antibody staining and flow cytometry Investigating intestinal immune responses is critical to understanding local and systemic immunity. However, obtaining resident intestinal immune cells with high cell viability can be challenging. Here, we provide an optimized protocol to isolate lamina propria lymphocytes from the small and large intestines, including lymphocyte activation for cytokine expression analysis and techniques for surface and intracellular antibody staining and flow cytometry. This protocol can be used for isolating and analyzing tissue-resident immune cells from other tissues with specified modifications.
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影响因子：
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作者：
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影响因子：
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影响因子：
7.9
作者：
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DOI：
10.1038/nri2515
发表时间：
2009-05
期刊：
Nature reviews. Immunology
影响因子：
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作者：
通讯作者：
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