Transcriptomic analyses reveal species-specific light-induced anthocyanin biosynthesis in chrysanthemum.
Transcriptomic analyses reveal species-specific light-induced anthocyanin biosynthesis in chrysanthemum.
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转录组分析揭示了菊花中物种特异性的光诱导花青素生物合成
DOI：
10.1186/s12864-015-1428-1
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发表时间：
2015-03-17
期刊：
影响因子：
4.4
通讯作者：
Dai S
中科院分区：
文献类型：
--
作者：
Hong Y;Tang X;Huang H;Zhang Y;Dai S
背景农产品花色是决定其商品价值的重要因素，花色苷的积累是决定花色的主要因素。光照是影响花色素苷生物合成的重要环境因子之一。然而，其深层次的分子机制仍不清楚，许多关于表型变化和相应的基因调控的问题仍不清楚。在本研究中，菊花×morifolium'紫里根'，一个光响应的色素沉着品种，选择光诱导的花青素的生物合成的机制进行了研究，采用转录组学analysis.ResultsOnly花青素衍生物被确定的基础上，在射线小花的色素沉着的分析。遮光试验表明，头状花序是关键器官，其芽期是对光反应的关键时期。这些结果被用来设计五个库的转录组学分析，包括三个头状花序发育阶段和两个光条件。RNA序列被重新组装成103，517个单基因，其中60，712个在四个公共蛋白质数据库中进行了注释。多达2，135个unigenes在光照和黑暗文库之间差异表达，其中923个上调和1，212个下调unigenes响应于遮蔽。接下来，交互作用途径分析表明，花青素生物合成途径是唯一的完整的代谢途径都调制响应于光和相关的头状花序的发展。遮荫处理后，几乎所有参与花青素生物合成途径的结构基因表达下调。通过聚类分析和RT-qPCR验证，确定了3个CmMYB基因和1个Cmb HLH基因可能是参与光照条件下花青素合成调控的关键转录因子。最后，推测菊花花色素苷的光诱导生物合成途径。结论菊花品种紫里根的射线小花的着色依赖于光。在光诱导的色素形成过程中，花青素生物合成途径中的7个结构基因的表达（受至少4种转录因子的调控）是菊花色素形成的主要贡献者。这些信息将有助于我们进一步了解光诱导观赏植物花色素苷生物合成的分子机制。
BackgroundThe flower colour of agricultural products is very important for their commercial value, which is mainly attributed to the accumulation of anthocyanins. Light is one of the key environmental factors that affect the anthocyanin biosynthesis. However, the deep molecular mechanism remains elusive, and many problems regarding the phenotypic change and the corresponding gene regulation are still unclear. In the present study,Chrysanthemum×morifolium‘Purple Reagan’, a light-responding pigmentation cultivar, was selected to investigate the mechanism of light-induced anthocyanin biosynthesis using transcriptomic analyses.ResultsOnly cyanidin derivatives were identified based on the analyses of the pigmentation in ray florets. Shading experiments revealed that the capitulum was the key organ and that its bud stage was the key phase responding to light. These results were used to design five libraries for transcriptomic analyses, including three capitulum developmental stages and two light conditions. RNA sequences werede novoassembled into 103,517 unigenes, of which 60,712 were annotated against four public protein databases. As many as 2,135 unigenes were differentially expressed between the light and dark libraries with 923 up-regulated and 1,212 down-regulated unigenes in response to shading. Next, interactive pathway analysis showed that the anthocyanin biosynthetic pathway was the only complete metabolic pathway both modulated in response to light and related to capitulum development. Following the shading treatment, nearly all structural genes involved in the anthocyanin biosynthetic pathway were down-regulated. Moreover, threeCmMYBgenes and oneCmbHLHgene were identified as key transcription factors that might participate in the regulation of anthocyanin biosynthesis under light conditions based on clustering analysis and validation by RT-qPCR. Finally, a light-induced anthocyanin biosynthesis pathway in chrysanthemums was inferred.ConclusionThe pigmentation of the ray florets of chrysanthemum cultivar ‘Purple Reagan’ is dependent on light. During the light-induced pigmentation process, the expression of seven structural genes in the anthocyanin biosynthetic pathway (regulated by at least four transcription factors in response to light) are the main contributors to the pigmentation of chrysanthemums. This information will further our understanding of the molecular mechanisms governing light-induced anthocyanin biosynthesis in ornamental plants.
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影响因子：
3.7
作者：
Hyun TK;Lee S;Rim Y;Kumar R;Han X;Lee SY;Lee CH;Kim JY
通讯作者：
Kim JY
影响因子：
4.4
作者：
Feng C;Chen M;Xu CJ;Bai L;Yin XR;Li X;Allan AC;Ferguson IB;Chen KS
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影响因子：
6.4
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作者：
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通讯作者：
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影响因子：
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作者：
Carletti, Giorgia;Lucini, Luigi;Bernardi, Jamila
通讯作者：
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