Survival and differentiation of transplanted neural stem cells derived from human induced pluripotent stem cells in a rat stroke model.
Survival and differentiation of transplanted neural stem cells derived from human induced pluripotent stem cells in a rat stroke model.
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DOI：
10.1016/j.jstrokecerebrovasdis.2011.09.008
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发表时间：
2013-05
期刊：
影响因子：
--
通讯作者：
Zhang SC
中科院分区：
文献类型：
--
作者：
Jensen MB;Yan H;Krishnaney-Davison R;Al Sawaf A;Zhang SC
尽管各种干细胞的施用在中风模型中显示出前景，但源自人类诱导多能干细胞 (iPSC) 的神经干细胞 (NSC) 比其他细胞类型具有优势。我们询问这些细胞是否能够在缺血性中风模型中存活、分化并改善中风恢复。人类 iPSC 在体外被诱导至早期 NSC 阶段。局灶性脑缺血1周后，20只大鼠通过脑内注射接受细胞或载体。评估移植细胞命运、梗塞体积和行为缺陷。在 8 只移植大鼠（80%）中发现了移植细胞，估计平均移植细胞数量几乎是一个月后移植数量的两倍。移植细胞还在 5 只大鼠（63%）中表达 NSC 标记，在所有 8 只大鼠（100%）中表达神经元，在 4 只大鼠（50%）中表达稀有星形胶质细胞，在 4 只大鼠（50%）中表达增殖迹象，但没有观察到肿瘤形成。各组之间的每搏量和行为恢复相似。据我们所知，这是第一份在中风模型中移植源自人类 iPSC 的 NSC 的报告。人类 iPSC 衍生的 NSC 在缺血后的大鼠大脑中存活，并且似乎主要分化为神经元。这种治疗中风的细胞移植方法似乎是可行的，但需要进一步优化。
Although administration of various stem cells has shown promise in stroke models, neural stem cells (NSCs) derived from human induced pluripotent stem cells (iPSCs) have advantages over other cell types. We asked if these cells could survive, differentiate, and improve stroke recovery in an ischemic stroke model. Human iPSCs were induced in vitro to an early NSC stage. One week after focal cerebral ischemia, 20 rats received cells or vehicle by intracerebral injection. Graft cell fate, infarct volume, and behavioral deficits were assessed. Graft cells were found in 8 of the transplanted rats (80%), with estimated mean graft cell numbers nearly double the amount transplanted one month later. Graft cells also expressed markers of NSCs in 5 rats (63%), neurons in all 8 rats (100%), rare astrocytes in 4 rats (50%), and signs of proliferation in 4 rats (50%), but no tumor formation was observed. Stroke volume and behavioral recovery were similar between groups. To our knowledge this is the first report of transplantation of NSCs derived from human iPSCs in a stroke model. Human iPSC-derived NSCs survived in the post-ischemic rat brain and appeared to differentiate, primarily into neurons. This cell transplantation approach for stroke appears to be feasible, but further optimization is needed.
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影响因子：
2.9
作者：
BORLONGAN, CV;CAHILL, DW;SANBERG, PR
通讯作者：
SANBERG, PR
影响因子：
8.3
作者：
Bliss, Tonya;Guzman, Raphael;Steinberg, Gary K.
通讯作者：
Steinberg, Gary K.
影响因子：
6.3
作者：
Kawai, Hiromi;Yamashita, Toru;Abe, Koji
通讯作者：
Abe, Koji
DOI：
10.1126/science.1172482
发表时间：
2009-05-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Yu J;Hu K;Smuga-Otto K;Tian S;Stewart R;Slukvin II;Thomson JA
通讯作者：
Thomson JA
影响因子：
4.3
作者：
Jiang, Mei;Lv, Lei;Dong, Qiang
通讯作者：
Dong, Qiang