Non-radioactively labelled DNA probes for the detection of periodontopathogenic Prevotella and Porphyromonas species.
Non-radioactively labelled DNA probes for the detection of periodontopathogenic Prevotella and Porphyromonas species.
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非放射性标记的 DNA 探针，用于检测牙周致病性普氏菌和卟啉单胞菌。
DOI：
10.1111/j.1574-695x.1993.tb00313.x
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发表时间：
1993
影响因子：
--
通讯作者：
A. Brauner
中科院分区：
文献类型：
--
作者：
G. Conrads;A. Brauner
应用DNA- rna斑点杂交技术，将地高辛标记的靶向16S rRNA的合成DNA探针用于龈下菌斑中牙周病致病菌普氏菌和牙龈卟啉单胞菌的直接检测。该试验对来自26例成人牙周炎或快速进行性牙周炎患者的134个菌斑样本进行了评估。检出下限为10(4)~ 10(5)个细菌/份。该技术对每个样品和相应牙周部位的两个物种进行了半定量评估。可以在两天内检测80-90个样品，材料成本低。
Digoxigenin-labelled synthetic DNA probes directed against the 16S rRNA were used for the direct detection of the periodontopathogenic bacteria Prevotella intermedia and Porphyromonas gingivalis in subgingival plaque by applying a DNA-RNA dot-blot hybridization procedure. The test was evaluated with 134 plaque samples from 26 patients with adult periodontitis or rapidly progressive periodontitis. The lower limit of detection was 10(4)-10(5) bacteria/specimen. A semiquantitative assessment of the two species in each sample and in the corresponding periodontal site was achieved by this technique. It is possible to examine 80-90 samples within two days with low material costs.
影响因子：
9.4
作者：
W. J. Loesche;D. Lopatin;'. J. Stoll;'. Neal;Van;Poperin;P. Hujoel
通讯作者：
W. J. Loesche;D. Lopatin;'. J. Stoll;'. Neal;Van;Poperin;P. Hujoel
影响因子：
56.9
作者：
HOLT, SC;EBERSOLE, J;KORNMAN, KS
通讯作者：
KORNMAN, KS
影响因子：
4.3
作者：
J. Zambon;H. Reynolds;R. Genco
通讯作者：
J. Zambon;H. Reynolds;R. Genco