Detection of Zika virus using reverse-transcription LAMP coupled with reverse dot blot analysis in saliva.
Detection of Zika virus using reverse-transcription LAMP coupled with reverse dot blot analysis in saliva.
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DOI：
10.1371/journal.pone.0192398
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Abrams WR
中科院分区：
文献类型：
--
作者：
Sabalza M;Yasmin R;Barber CA;Castro T;Malamud D;Kim BJ;Zhu H;Montagna RA;Abrams WR
In recent years, there have been increasing numbers of infectious disease outbreaks that spread rapidly to population centers resulting from global travel, population vulnerabilities, environmental factors, and ecological disasters such as floods and earthquakes. Some examples of the recent outbreaks are the Ebola epidemic in West Africa, Middle East respiratory syndrome coronavirus (MERS-Co) in the Middle East, and the Zika outbreak through the Americas. We have created a generic protocol for detection of pathogen RNA and/or DNA using loop-mediated isothermal amplification (LAMP) and reverse dot-blot for detection (RDB) and processed automatically in a microfluidic device. In particular, we describe how a microfluidic assay to detect HIV viral RNA was converted to detect Zika virus (ZIKV) RNA. We first optimized the RT-LAMP assay to detect ZIKV RNA using a benchtop isothermal amplification device. Then we implemented the assay in a microfluidic device that will allow analyzing 24 samples simultaneously and automatically from sample introduction to detection by RDB technique. Preliminary data using saliva samples spiked with ZIKV showed that our diagnostic system detects ZIKV RNA in saliva. These results will be validated in further experiments with well-characterized ZIKV human specimens of saliva. The described strategy and methodology to convert the HIV diagnostic assay and platform to a ZIKV RNA detection assay provides a model that can be readily utilized for detection of the next emerging or re-emerging infectious disease.
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影响因子：
30.5
作者：
Dejnirattisai W;Supasa P;Wongwiwat W;Rouvinski A;Barba-Spaeth G;Duangchinda T;Sakuntabhai A;Cao-Lormeau VM;Malasit P;Rey FA;Mongkolsapaya J;Screaton GR
通讯作者：
Screaton GR
影响因子：
3.8
作者：
Bonaldo MC;Ribeiro IP;Lima NS;Dos Santos AA;Menezes LS;da Cruz SO;de Mello IS;Furtado ND;de Moura EE;Damasceno L;da Silva KA;de Castro MG;Gerber AL;de Almeida LG;Lourenço-de-Oliveira R;Vasconcelos AT;Brasil P
通讯作者：
Brasil P
影响因子：
--
作者：
Malamud D
通讯作者：
Malamud D
DOI：
10.1073/pnas.1522305113
发表时间：
2016-06-07
影响因子：
11.1
作者：
Finger, Flavio;Genolet, Tina;Bertuzzo, Enrico
通讯作者：
Bertuzzo, Enrico
影响因子：
158.5
作者：
Driggers, R. W.;Ho, C. -Y.;Vapalahti, O.
通讯作者：
Vapalahti, O.