MKK3 was involved in larval settlement of the barnacle Amphibalanus amphitrite through activating the kinase activity of p38MAPK.
MKK3 was involved in larval settlement of the barnacle Amphibalanus amphitrite through activating the kinase activity of p38MAPK.
复制标题
DOI：
10.1371/journal.pone.0069510
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Qian PY
中科院分区：
文献类型：
--
作者：
Zhang G;He LS;Wong YH;Qian PY
p38丝裂原活化蛋白激酶（p38MAPK）在藤壶Amphibalanus amphitrite幼虫定居过程中起着关键作用。为了研究p38MAPK在幼虫附着过程中的信号通路，我们试图鉴定p38MAPK的上游激酶。在A. amphitrite的基因克隆并在E.杆菌通过激酶分析，我们发现MKK3磷酸化p38MAPK，而不是MKK4或MKK7。此外，MKK3活性对p38MAPK是特异性的，因为它不磷酸化ERK或JNK。为了进一步研究MKK3和p38 MAPK在体内的功能关系，我们用免疫组化的方法研究了磷酸化MKK3（pMKK3）和MKK3的定位。与p38MAPK和磷酸化p38MAPK（pp38MAPK）的模式一致，pMKK 3和MKK 3主要定位于Cyprids的触角。蛋白质印迹分析显示，与无节幼体和幼年期相比，pMKK3水平（如pp38 MAPK水平）在塞浦路斯期升高。此外，pMKK3水平增加后处理与成年藤壶粗提物，表明MKK3可能介导的刺激作用的成年藤壶提取物对p38 MAPK通路。
The p38 mitogen-activated protein kinase (p38MAPK) plays a key role in larval settlement of the barnacle Amphibalanus amphitrite. To study the signaling pathway associated with p38MAPK during larval settlement, we sought to identify the upstream kinase of p38MAPK. Three MKKs (MKK3, MKK4 and MKK7) and three MAPKs (p38MAPK, ERK and JNK) in A. amphitrite were cloned and recombinantly expressed in E. coli. Through kinase assays, we found that MKK3, but not MKK4 or MKK7, phosphorylated p38MAPK. Furthermore, MKK3 activity was specific to p38MAPK, as it did not phosphorylate ERK or JNK. To further investigate the functional relationship between MKK3 and p38MAPK in vivo, we studied the localization of phospho-MKK3 (pMKK3) and MKK3 by immunostaining. Consistent with the patterns of p38MAPK and phospho-p38MAPK (pp38MAPK), pMKK3 and MKK3 mainly localized to the antennules of the cyprids. Western blot analysis revealed that pMKK3 levels, like pp38MAPK levels, were elevated at cyprid stage, compared to nauplii and juvenile stages. Moreover, pMKK3 levels increased after treatment with adult barnacle crude extracts, suggesting that MKK3 might mediate the stimulatory effects of adult barnacle extracts on the p38MAPK pathway.
登录
查看更多内容
影响因子：
3.7
作者：
Chen ZF;Matsumura K;Wang H;Arellano SM;Yan X;Alam I;Archer JA;Bajic VB;Qian PY
通讯作者：
Qian PY
影响因子：
56.9
作者：
Ge, BX;Gram, H;Han, JH
通讯作者：
Han, JH
影响因子：
3.5
作者：
DOZA, YN;CUENDA, A;NEBREDA, AR
通讯作者：
NEBREDA, AR
影响因子：
11.4
作者：
Inoue, H;Tateno, M;Matsumoto, K
通讯作者：
Matsumoto, K
影响因子：
10.5
作者：
Brancho, D;Tanaka, N;Davis, RJ
通讯作者：
Davis, RJ