Studying 18 MDa virus assemblies with native mass spectrometry.
Studying 18 MDa virus assemblies with native mass spectrometry.
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DOI：
10.1002/anie.201210197
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发表时间：
2013-04-02
影响因子：
16.6
通讯作者：
Heck, Albert J. R.
中科院分区：
文献类型：
--
作者：
Snijder, Joost;Rose, Rebecca J.;Veesler, David;Johnson, John E.;Heck, Albert J. R.
继John Fenn的诺贝尔奖获得者演讲（“分子电喷雾翼”）之后，[1]天然质谱（MS）已被开发为研究大型非共价蛋白质复合物的强大分析技术。[2]使用软纳米电喷雾电离（nESI），这些蛋白质复合物可以完整地转移到气相中，而不会显着损失四级或三级结构。市售MS仪器的微小修改[3]可以大大提高大离子的传输，因此可以对完整蛋白质复合物进行准确和精确的质量分析。因此，蛋白质复合物的化学计量可以在天然MS方法中容易地确定。溶液相和气相解离的研究沿着MS与离子迁移率的结合，也可以获得有关蛋白质复合物稳定性和拓扑结构的有价值的信息。[4]天然MS先前已被应用于完整病毒和病毒衣壳的研究。[5]除了它们在病毒发病机制中的作用外，衣壳还因为它们在纳米技术和医学中作为纳米容器和纳米反应器以及作为组装支架的潜在应用而引起人们的兴趣。[6]天然MS使得能够研究完整的病毒衣壳，其中定量（非天然）货物包封并监测衣壳组装。[7]这些先前的研究集中在相对较小的衣壳上，卡斯帕-克鲁格三角测量数T= 1，3和4，质量范围高达10 MDa。虽然可以用MS检测到10 MDa诺如病毒衣壳，但由于缺乏单个电荷状态的分辨率，无法确定其精确质量。[7b]电荷分辨光谱仅限于高达约5 MDa的颗粒，这被假设为可实现的尺寸上限。
Following John Fenn s Nobel Laureate lecture (“Electrospray Wings for Molecular Elephants”),[1] native mass spectrometry (MS) has been developed as a powerful analytical technique to study large noncovalent protein complexes.[2] Using soft nano-electrospray ionization (nESI), these protein complexes can be transferred intact into the gas phase without significant loss of quaternary or tertiary structure. Minor modifications of commercially available MS instruments [3] can greatly improve the transmission of large ions, therefore yielding accurate and precise mass analysis of intact protein complexes. As a result, the stoichiometry of protein complexes can be readily determined in a native MS approach. The study of solution-phase and gas-phase dissociation along with the combination of MS with ion mobility can also yield valuable information on the stability and topology of a protein complex.[4]Native MS has previously been applied to the study of intact viruses and virus capsids.[5] Apart from their role in viral pathogenesis, capsids are interesting because of their potential applications in nanotechnology and medicine as nanocontainers and nanoreactors and as assembly scaffolds.[6] Native MS enabled the study of intact virus capsids in which (nonnative) cargo encapsulation was quantified and capsid assembly was monitored.[7] These previous studies focused on relatively small capsids with Caspar–Klug triangulation numbers of T= 1, 3, and 4, ranging up to 10 MDa in mass. Although the 10 MDa norovirus capsids could be detected with MS, their precise mass could not be determined due to a lack of resolution of individual charge states.[7b] Chargeresolved spectra were limited to particles up to roughly 5 MDa, which was hypothesized to be the upper size limit achievable.
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影响因子：
1.8
作者：
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作者：
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作者：
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