Developmental profiling of postnatal dentate gyrus progenitors provides evidence for dynamic cell-autonomous regulation.
Developmental profiling of postnatal dentate gyrus progenitors provides evidence for dynamic cell-autonomous regulation.
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DOI：
10.1002/hipo.20719
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发表时间：
2011-01
期刊：
影响因子：
3.5
通讯作者：
Kernie, Steven G.
中科院分区：
文献类型：
--
作者：
Gilley, Jennifer A.;Yang, Cui-Ping;Kernie, Steven G.
海马的齿状回是哺乳动物出生后大脑中最重要的区域之一，在那里神经发生持续一生。关于成年海马神经发生的潜在影响有很多猜测，尽管目前还不清楚这种能力在正常衰老过程中会减弱到什么程度，以及随着时间的推移，祖细胞群体中会发生什么样的遗传变化。使用巢蛋白启动子的定义元素，我们开发了一种转基因小鼠，可靠地标记神经干细胞和早期祖细胞与绿色荧光蛋白（GFP）。使用免疫组织化学和流式细胞术技术相结合，我们的特点是祖细胞内的齿状回，并创建了一个发育概况，从出生后第7天（P7），直到6个月的年龄。此外，我们证明，这些祖细胞的增殖潜力至少部分由细胞自主线索控制。最后，为了确定这些差异的潜在原因，我们对分离的P7和出生后第28天（P28）齿状回的GFP表达分选细胞进行了干细胞特异性微阵列。我们确定了几个差异表达的基因，这些基因可能是我们在这些不同年龄的分选细胞和分化试验的神经球测定中观察到的功能差异的基础。这些数据表明，从齿状回的神经祖细胞的差异调节细胞自主的因素，随着时间的推移而变化。
The dentate gyrus of the hippocampus is one of the most prominent regions in the postnatal mammalian brain where neurogenesis continues throughout life. There is tremendous speculation regarding the potential implications of adult hippocampal neurogenesis, though it remains unclear to what extent this ability becomes attenuated during normal aging, and what genetic changes in the progenitor population ensue over time. Using defined elements of the nestin promoter, we developed a transgenic mouse that reliably labels neural stem and early progenitors with green fluorescent protein (GFP). Using a combination of immunohistochemical and flow cytometry techniques, we characterized the progenitor cells within the dentate gyrus and created a developmental profile from postnatal day 7 (P7) until 6 months of age. In addition, we demonstrate that the proliferative potential of these progenitors is controlled at least in part by cell-autonomous cues. Finally, in order to identify what may underlie these differences, we performed stem cell-specific microarrays on GFP-expressing sorted cells from isolated P7 and postnatal day 28 (P28) dentate gyrus. We identified several differentially expressed genes that may underlie the functional differences that we observe in neurosphere assays from sorted cells and differentiation assays at these different ages. These data suggest that neural progenitors from the dentate gyrus are differentially regulated by cell-autonomous factors that change over time.
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影响因子：
2.5
作者：
Bulloch, Karen;Miller, Melinda M.;McEwen, Bruce S.
通讯作者：
McEwen, Bruce S.
影响因子：
7.8
作者：
Bailey, KJ;Maslov, AY;Pruitt, SC
通讯作者：
Pruitt, SC
影响因子：
1.7
作者：
Armando, Sylvain;Lebrun, Aurore;Simonneau, Lionel
通讯作者：
Simonneau, Lionel
影响因子：
5.7
作者：
Calof, Anne L.
通讯作者：
Calof, Anne L.
DOI：
10.1073/pnas.0601992103
发表时间：
2006-05-23
影响因子：
11.1
作者：
Encinas, Juan M.;Vaahtokari, Anne;Enikolopov, Grigori
通讯作者：
Enikolopov, Grigori