Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography.
Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography.
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DOI：
10.1083/jcb.200606007
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发表时间：
2006-09-11
期刊：
影响因子：
--
通讯作者：
Kusumi A
中科院分区：
文献类型：
--
作者：
Morone N;Fujiwara T;Murase K;Kasai RS;Ike H;Yuasa S;Usukura J;Kusumi A
通过电子断层扫描重建正常大鼠肾成纤维细胞（NRK）细胞和胎鼠皮肤角质形成细胞上细胞膜细胞质表面上的底衣结构的三维图像，使用快速冷冻、深度蚀刻、铂复制的质膜从细胞质表面连续制作0.85 nm厚的切片。膜骨架（MSK）主要由肌动蛋白丝和相关蛋白组成。MSK覆盖整个细胞质表面，并与网格蛋白包被的小凹和小窝密切相关。与质膜的细胞质表面紧密贴壁的肌动蛋白丝（在10.2 nm内）可能形成负责暂时限制膜分子的膜隔室的边界，从而在其横向扩散方面划分质膜。由电子断层扫描确定的MSK网格大小的分布和由高速单颗粒跟踪磷脂扩散确定的隔室大小的分布在两种细胞类型中一致，支持MSK栅栏和MSK锚定蛋白质尖桩模型。
Three-dimensional images of the undercoat structure on the cytoplasmic surface of the upper cell membrane of normal rat kidney fibroblast (NRK) cells and fetal rat skin keratinocytes were reconstructed by electron tomography, with 0.85-nm–thick consecutive sections made ∼100 nm from the cytoplasmic surface using rapidly frozen, deeply etched, platinum-replicated plasma membranes. The membrane skeleton (MSK) primarily consists of actin filaments and associated proteins. The MSK covers the entire cytoplasmic surface and is closely linked to clathrin-coated pits and caveolae. The actin filaments that are closely apposed to the cytoplasmic surface of the plasma membrane (within 10.2 nm) are likely to form the boundaries of the membrane compartments responsible for the temporary confinement of membrane molecules, thus partitioning the plasma membrane with regard to their lateral diffusion. The distribution of the MSK mesh size as determined by electron tomography and that of the compartment size as determined from high speed single-particle tracking of phospholipid diffusion agree well in both cell types, supporting the MSK fence and MSK-anchored protein picket models.
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DOI：
10.1083/jcb.103.2.351
发表时间：
1986-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Apgar JR;Mescher MF
通讯作者：
Mescher MF
影响因子：
5.6
作者：
HEUSER, JE
通讯作者：
HEUSER, JE
影响因子：
56.9
作者：
EDIDIN, M;KUO, SC;SHEETZ, MP
通讯作者：
SHEETZ, MP
影响因子：
3
作者：
Kremer, JR;Mastronarde, DN;McIntosh, JR
通讯作者：
McIntosh, JR
DOI：
10.1083/jcb.91.2.399
发表时间：
1981-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hirokawa N;Heuser JE
通讯作者：
Heuser JE