Agorins: major structural proteins of the plasma membrane skeleton of P815 tumor cells.
Agorins: major structural proteins of the plasma membrane skeleton of P815 tumor cells.
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DOI：
10.1083/jcb.103.2.351
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发表时间：
1986-08
期刊：
影响因子：
--
通讯作者：
Mescher MF
中科院分区：
文献类型：
--
作者：
Apgar JR;Mescher MF
P815肥大细胞瘤细胞的质膜含有一组蛋白质，这些蛋白质在用Triton X-100提取膜后保持选择性不溶，并且似乎形成独立于丝状细胞骨架系统的膜骨架基质。EGTA处理的基质被发现释放约25%的蛋白质作为多肽的70，69，38，和36 kD，所有这些似乎是周边组件与质膜的细胞质面通过二价阳离子依赖性相互作用。约75%的总基质蛋白在EGTA不溶性级分中回收。肌动蛋白占EGTA不溶性级分中总蛋白质的约5%。其余的占两个新的蛋白质的20和40 kD，尽管它们的分子量相对较低，不进入SDS PAGE凝胶。这些蛋白质一起占总质膜蛋白质的约15%，因此以比有核细胞质膜的任何其他特征蛋白质高得多的量存在。基于这些蛋白质的广泛协会，形成非常大的洗涤剂不溶性结构，我们建议，他们可能会被命名为agorin I，20 kD的蛋白质，和agorin II，40 kD的蛋白质，从希腊agora的意思组装。这些蛋白质的量和性质以及EGTA不溶性物质在薄切片电子显微照片中的出现表明，聚集蛋白是膜基质的主要结构元素，因此是推定的膜骨架的主要结构元素。
Plasma membranes of P815 mastocytoma cells contain a set of proteins that remain selectively insoluble upon extraction of the membranes with Triton X-100, and appear to form a membrane skeletal matrix independent of the filamentous cytoskeletal systems. EGTA treatment of the matrix was found to release approximately 25% of the protein as polypeptides of 70, 69, 38, and 36 kD, all of which appear to be peripheral components associated with the cytoplasmic face of the plasma membrane via divalent cation-dependent interactions. About 75% of the total matrix protein was recovered in the EGTA-insoluble fraction. Actin accounted for approximately 5% of the total protein in the EGTA- insoluble fraction. The rest was accounted for by two novel proteins of 20 and 40 kD which, despite their relatively low molecular weights, do not enter SDS PAGE gels. Together these proteins account for approximately 15% of the total plasma membrane protein, and are thus present in much higher amounts than any other characterized protein of nucleated cell plasma membranes. Based on the extensive associations of these proteins to form very large detergent-insoluble structures, we propose that they may be named agorin I, the 20-kD protein, and agorin II, the 40-kD protein, from the Greek agora meaning assembly. The amount and properties of these proteins and the appearance of the EGTA- insoluble material in thin-section electron micrographs indicate that the agorins are the major structural elements of the membrane matrix, and thus of the putative membrane skeleton.
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DOI：
10.1083/jcb.91.1.103
发表时间：
1981-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fulton AB;Prives J;Farmer SR;Penman S
通讯作者：
Penman S
影响因子：
3.7
作者：
LEHTO, VP;VARTIO, T;VIRTANEN, I
通讯作者：
VIRTANEN, I
影响因子：
11.4
作者：
OWENS, RJ;GALLAGHER, CJ;CRUMPTON, MJ
通讯作者：
CRUMPTON, MJ
DOI：
10.1016/0005-2736(71)90331-2
发表时间：
1971-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
AVRUCH, J;WALLACH, DFH
通讯作者：
WALLACH, DFH
影响因子：
5.6
作者：
HENDERSON, D;EIBL, H;WEBER, K
通讯作者：
WEBER, K