Amelioration of reproduction-associated oxidative stress in a viviparous insect is critical to prevent reproductive senescence.
Amelioration of reproduction-associated oxidative stress in a viviparous insect is critical to prevent reproductive senescence.
复制标题
DOI：
10.1371/journal.pone.0087554
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Aksoy S
中科院分区：
文献类型：
--
作者：
Michalkova V;Benoit JB;Attardo GM;Medlock J;Aksoy S
生殖过程对女性健康的影响产生了相互矛盾的结果;特别是在与生殖有关的压力的作用方面。我们使用胎生采采蝇来确定哺乳、出生和退化是否会导致氧化应激（OS）损伤，从而损害随后的生殖周期。采采蝇雌性在每个怀孕周期都携带一个子宫内的幼虫到足月，并通过副腺（=乳腺）器官产生的乳汁分泌物来喂养它们。 与大多数K策略者不同，采采蝇雌性缺乏明显的生殖衰老期，在其整个生命周期内可以产生8-10个后代。在哺乳期女性中，超过47%的母体转录组与乳蛋白的产生相关。由此产生的单个幼虫后代的体重与母亲出生时一样重。在研究这一过程中，我们注意到在泌乳、分娩和乳腺/脂肪体器官和子宫退化的关键时期，特定抗氧化酶（AOE）转录本和酶活性增加。抑制超氧化物歧化酶（sod）会降低年轻母亲在随后的生殖周期中的生育能力，而几乎会消除老年女性的生育能力。繁殖力的丧失部分是由于母亲无法产生足够的乳汁来支持幼虫的生长。在草皮被推倒后，寿命也受到了影响。在与妊娠间隔相对应的时间内，用过氧化氢进行外源性处理，在处女雌性中产生OS，降低了存活率，这通过sod敲除而加剧。AOE表达可以防止与通过乳腺、分娩和乳腺分解产生营养物相关的氧化损伤。我们的研究结果表明，预防OS是必不可少的女性，以满足日益增长的营养需求的青少年在怀孕期间，并修复在出生时发生的损害。这一过程对于女性在其生命的后半部分保持生育能力特别重要。
Impact of reproductive processes upon female health has yielded conflicting results; particularly in relation to the role of reproduction-associated stress. We used the viviparous tsetse fly to determine if lactation, birth and involution lead to damage from oxidative stress (OS) that impairs subsequent reproductive cycles. Tsetse females carry an intrauterine larva to full term at each pregnancy cycle, and lactate to nourish them with milk secretions produced by the accessory gland ( = milk gland) organ. Unlike most K-strategists, tsetse females lack an apparent period of reproductive senescence allowing the production of 8–10 progeny over their entire life span. In a lactating female, over 47% of the maternal transcriptome is associated with the generation of milk proteins. The resulting single larval offspring weighs as much as the mother at birth. In studying this process we noted an increase in specific antioxidant enzyme (AOE) transcripts and enzymatic activity at critical times during lactation, birth and involution in the milk gland/fat body organ and the uterus. Suppression of superoxide dismutase (sod) decreased fecundity in subsequent reproductive cycles in young mothers and nearly abolished fecundity in geriatric females. Loss of fecundity was in part due to the inability of the mother to produce adequate milk to support larval growth. Longevity was also impaired after sod knockdown. Generation of OS in virgin females through exogenous treatment with hydrogen peroxide at times corresponding to pregnancy intervals reduced survival, which was exacerbated by sod knockdown. AOE expression may prevent oxidative damage associated with the generation of nutrients by the milk gland, parturition and milk gland breakdown. Our results indicate that prevention of OS is essential for females to meet the growing nutritional demands of juveniles during pregnancy and to repair the damage that occurs at birth. This process is particularly important for females to remain fecund during the latter portion of their lifetime.
登录
查看更多内容
影响因子：
--
作者：
Back P;Braeckman BP;Matthijssens F
通讯作者：
Matthijssens F
影响因子：
3.8
作者：
Attardo GM;Benoit JB;Michalkova V;Patrick KR;Krause TB;Aksoy S
通讯作者：
Aksoy S
DOI：
10.1093/gerona/53a.4.b245
发表时间：
1998-07-01
影响因子：
5.1
作者：
Carey, JR;Liedo, P;Chiou, JM
通讯作者：
Chiou, JM
影响因子：
56.9
作者：
Carey, JR;Liedo, P;Vaupel, JW
通讯作者：
Vaupel, JW
影响因子：
2.1
作者：
Dao A;Kassogue Y;Adamou A;Diallo M;Yaro AS;Traore SF;Lehmann T
通讯作者：
Lehmann T