Rehydration outcomes for freeze-dried red blood cells in reduced gravity
Rehydration outcomes for freeze-dried red blood cells in reduced gravity
复制标题
减重状态下冻干红细胞的补液结果
DOI：
10.1016/j.actaastro.2023.10.006
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发表时间：
2024
影响因子：
3.5
通讯作者：
Menze, Michael A.
中科院分区：
文献类型：
--
作者：
Elder, Charles A.;Moore, John;Janis, Brett R.;Shacklette, Sienna;Jones, Clara;Cantrell, Ryan;Grimm, David F.;Pantalos, George;Kopechek, Jonathan A.;Menze, Michael A.
太空探索的医疗计划是基于“浮动”血库模式，以储存救命的红细胞（RBC）用于紧急情况。在多名宇航员患有太空贫血症的情况下，“漂浮”血库的办法是不够的。在这些情况下，长期保存的红细胞对于保证船员的健康和安全至关重要。可转移的RBC单位只能冷藏42天或在−80 °C下冷冻。然而，在长期太空飞行的密闭条件下，将冷冻红细胞储存在-80 °C下具有挑战性。冷冻干燥的活红细胞将是一个合适的替代品，因为它们可以在不冷却的情况下储存，预计保质期为数年，并且可以在补液后立即输血。这项研究探讨了冷冻干燥的红细胞是否可以在重力降低的情况下再水化和输血，并在地球重力下观察到类似的恢复结果。实验分析冻干红细胞回收率，补液流体动力学，输血流量进行了分析，利用实验手套箱在模拟0 g抛物线飞行。在模拟0 g中，5 mL和10 mL体积的RBC回收率和再水化流体动力学与在1 g中获得的结果相同。缓慢静脉输注和快速液体复苏的临床可接受的流速范围是可能的，简单地增加一个手泵临床压力袋周围的一个单位的再水化红细胞。研究结果表明，在深空探测期间使用冻干细胞进行医疗保健具有潜在的可行性。
Medical planning for space exploration is based on the “floating” blood bank model to store life-saving red blood cells (RBCs) for emergencies. The “floating” blood bank approach is not sufficient in cases where multiple crewmembers are affected by space anemia. In these situations, long-term preserved RBCs will be vital to guarantee the health and safety of crew members. Transfusable RBC units can only be refrigerated for 42 days or frozen at −80 °C. However, storing frozen RBCs at −80 °C is challenging during the confined condition of long-duration space flight. Freeze-dried, viable RBCs would be an appropriate alternative because they can be stored without cooling, are predicted to have a shelf-life of years, and could be transfused immediately after rehydration. This study explores if freeze-dried RBCs can be rehydrated and transfused in reduced gravity with similar outcomes in recovery as observed at Earth gravity. Experiments analyzing freeze-dried RBC recoveries, rehydration fluid dynamics, and transfusion flow rates were analyzed utilizing an experimental glovebox in simulated 0gduring parabolic flights. RBC recoveries and rehydration fluid dynamics for volumes of 5 mL and 10 mL were the same in simulated 0gcompared to results obtained at 1g. A clinically acceptable range of flow rates for slow intravenous infusion and rapid fluid resuscitation was possible with the simple augmentation of a hand-pumped clinical pressure bag around a unit of rehydrated RBCs. The results demonstrate the potential feasibility of using freeze-dried cells for healthcare during deep-space exploration.
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影响因子：
2
作者：
Osaro E;Charles AT
通讯作者：
Charles AT
影响因子：
2.9
作者：
Ramakrishnan VT;Cattamanchi S
通讯作者：
Cattamanchi S
DOI：
10.1073/pnas.89.3.967
发表时间：
1992-02-01
影响因子：
11.1
作者：
GOODRICH, RP;SOWEMIMOCOKER, SO;TANAKA, KR
通讯作者：
TANAKA, KR
影响因子：
--
作者：
J. Crowe
通讯作者：
J. Crowe
DOI：
10.1016/s1095-6433(01)00505-0
发表时间：
2002-03-01
影响因子：
2.3
作者：
Wolkers, WF;Tablin, F;Crowe, JH
通讯作者：
Crowe, JH