Evaluation of raw and processed Phellodendri Chinensis Cortex using the quality marker analysis strategy by UHPLC-Q-Orbitrap MS and multivariate statistical analysis.
Evaluation of raw and processed Phellodendri Chinensis Cortex using the quality marker analysis strategy by UHPLC-Q-Orbitrap MS and multivariate statistical analysis.
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采用超高效液相色谱 - 四极杆 - 轨道阱质谱（UHPLC - Q - Orbitrap MS）结合多元统计分析的质量标志物分析策略对黄柏生药材及炮制品进行评价
DOI：
10.3389/fchem.2023.1223865
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发表时间：
2023
影响因子：
5.5
通讯作者：
中科院分区：
文献类型：
--
作者：
引言：黄柏因其显著的临床疗效是医疗保健的必要部分。生黄柏和炮制品均收录于《中国药典》（2015年版）。根据中医药理论，炮制后其治疗效果被认为有所不同。然而，依据中医药理论，这种炮制所涉及的化学机制仍不明确。
方法：本研究基于中医药理论，采用超高效液相色谱 - 四极杆 - 轨道阱高分辨质谱联用技术（UHPLC - Q - Orbitrap MS），结合主成分分析（PCA）和正交偏最小二乘法判别分析（OPLS - DA）等多元统计分析方法，对各种离子的治疗效果进行检测，首次全面比较生黄柏和炮制品之间的差异。
结果：共筛选出48种化合物，其中10种在炮制品中与原料相比发生显著变化且同时发生转化。结果表明，小檗碱、巴马汀、药根碱、木兰花碱、蝙蝠葛碱、黄柏碱、四氢药根碱和四氢巴马汀的含量降低，而小檗红碱和阿魏酰奎尼酸甲酯这两种化合物在炮制品中新增。这可能与炮制过程中成分的转化有关。
讨论：总之，已成功鉴定出质量标志物以区分黄柏炮制品和原料这一事实表明，该方法可用于研究草药炮制所涉及的化学转化机制。
Introduction: Phellodendri Chinensis Cortex is a necessary part of healthcare for its significant clinical efficacy. Raw and processed Phellodendri Chinensis Cortex is both documented in the Chinese Pharmacopoeia (2015). After processing, the therapeutic effects are believed to differ according to traditional Chinese medicine theories. However, the chemical mechanism responsible for this processing, according to traditional Chinese medicine theories, is still not clear. Methods: In this study, the therapeutic effects of various ions were examined based on traditional Chinese medicine theories by ultra-high performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometry (UHPLC-Q-Orbitrap MS) coupled with multivariate statistical analysis, such as principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), to comprehensively compare the differences between raw and processed Phellodendri Chinensis Cortex for the first time. Results: A total of 48 compounds were screened, out and 10 of them simultaneously transformed with significant variation in processed products compared with raw materials. It was illustrated that the contents of berberine, palmatine, jatrorrhizine, magnoflorine, menisperine, phellodendrine, tetrahydrojatrorrhizine, and tetrahydropalmatine decreased, while the compounds of berberrubine and fernloylquinic acid methyl ester newly appeared in processed herbs. This is likely to be related to the conversion of ingredients during processing. Discussion: Altogether, the fact that quality markers have been successfully identified to differentiate processed Phellodendri Chinensis Cortex from raw materials suggests that this approach could be used for the investigation of chemical transformation mechanisms involved in the processing of herbal medicine.
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影响因子：
3.1
作者：
Ren, Lingling;Xue, Xingya;Liang, Xinmiao
通讯作者：
Liang, Xinmiao
影响因子：
2
作者：
Price, Erin R.;McClure, Pamela J.;Espinoza, Edgard O.
通讯作者：
Espinoza, Edgard O.
影响因子：
3.1
作者：
Xian, Xiaoyan;Sun, Bohang;Gao, Huiyuan
通讯作者：
Gao, Huiyuan
DOI：
10.1016/j.jpba.2004.11.026
发表时间：
2005-03-09
影响因子：
3.4
作者：
Wu, W;Song, FR;Liu, SY
通讯作者：
Liu, SY
多元分析法对黄柏和黄柏的化学成分鉴别
影响因子：
0.7
作者：
Sun H;Wang H;Zhang A;Yan G;Han Y;Li Y;Wu X;Meng X;Wang X
通讯作者：
Wang X